油茶粕多糖的分级纯化及结构研究

doi:10.7506/spkx1002-6630-201323021

食品科学 ›› 2013, Vol. 34 ›› Issue (23): 96-102.doi: 10.7506/spkx1002-6630-201323021

油茶粕多糖的分级纯化及结构研究

刘小如，张丽美，胡蒋宁，刘蓉，李红艳，范亚苇，李静，邓泽元*

南昌大学食品科学与技术国家重点实验室，南昌大学生命科学与食品工程学院，南昌大学高等研究院，江西南昌 330047

收稿日期:2013-07-20 修回日期:2013-10-30 出版日期:2013-12-15 发布日期:2014-01-03
通讯作者: 邓泽元 E-mail:dengzy@ncu.edu.cn
基金资助:
科技部星火计划协作项目(2011GA730001)；2010年江西省重大科技专项(赣科发[2010]217号)

Fractional Purification and Structural Analysis of Polysaccharides Extracted from Camellia oleifera Seed Cake

LIU Xiao-ru，ZHANG Li-mei，HU Jiang-ning，LIU Rong，LI Hong-yan，FAN Ya-wei，LI Jing，DENG Ze-yuan*

State Key Laboratory of Food Science and Technology, College of Life Science and Food Engineering,
Institute of Advanced Studies, Nanchang University, Nanchang 330047, China

Received:2013-07-20 Revised:2013-10-30 Online:2013-12-15 Published:2014-01-03
Contact: DENG Ze-Yuan E-mail:dengzy@ncu.edu.cn

摘要/Abstract

摘要：

目的：分级纯化油茶粕多糖(CSP)，并对纯化组分进行结构分析。方法：脱蛋白后的油茶粕多糖透析冻干，再经DEAE-Sephadex A-25阴离子交换树脂柱层析分级纯化，并以紫外扫描光谱、Sephacryl S-200葡聚糖凝胶柱层析和HPLC测定所得不同多糖组分的纯度；采用柱前衍生化HPLC法分析单糖组成，再以红外光谱扫描及核磁共振对多糖构型进行初步分析。结果：分级纯化得CSP1、CSP2和CSP3 3个多糖组分，经鉴定CSP1和CSP2为中性多糖，CSP3为酸性多糖；纯度测定表明CSP1和CSP2是杂多糖，而CSP3是均一多糖；CSP1的单糖组成为葡萄糖、甘露糖及鼠李糖，物质的量比为6.29:1.00:0.15；CSP2的单糖组成为葡萄糖、甘露糖和半乳糖，物质的量比为3.13:1.00:0.03；CSP3的单糖组成为葡萄糖、甘露糖、半乳糖、阿拉伯糖、半乳糖醛酸和少量鼠李糖，物质的量比为0.42:1.00:1.09:0.96:0.43:0.19。

关键词: 油茶粕, 多糖, 分级纯化, 结构

Abstract:

In this study, we conducted fractional purification and structure analysis of polysaccharides (CSPs) extracted
from Camellia oleifera (C. oleifera) seed cake with the aim to provide experimental data for subsequent studies on the
structure-function relationship of CSPs. Methods: After removal of proteins, the crude CSPs were dialyzed, and then
fractionated and purified by DEAE-Sephadex A-25 anion exchange resin column chromatography. UV spectroscopy,
Sephacryl S-200 column chromatography and HPLC were used to detect the purity of the purified polysaccharides. A precolumn
derivatization HPLC method was used to analyze the monosaccharide composition. Infrared spectroscopy and a
nuclear magnetic resonance scanner were then used to study the configuration and structures of the purified CSPs. The
fractional purification led to three polysaccharides, namely CSP1, CSP2 and CSP3, as the main polysaccharides of CSP.
Both CSP1 and CSP2 were found to be neutral heteropolysaccharides whereas CSP3 a homogeneous, acidic one. CSP1was
composed of glucose, mannose and rhamnose with a molar ratio of 6.29:1.00:0.15; CSP2 was composed of monosaccharides
glucose, mannose and galactose with amolar ratio of 3.13:1.00:0.03; and CSP3 was composed of monosaccharides glucose,
mannose, galactose, arabinose, galactose, rhamnose, galacturonic acid and a small amount of rhamnose with a molar ratio of
0.42:1.00:1.09:0.96:0.43:0.19.

Key words: Camellia oleifera seed cake, polysaccharides, fractional purification, structure

中图分类号:

Q929.2

刘小如，张丽美，胡蒋宁，刘蓉，李红艳，范亚苇，李静，邓泽元*. 油茶粕多糖的分级纯化及结构研究[J]. 食品科学, 2013, 34(23): 96-102.

LIU Xiao-ru，ZHANG Li-mei，HU Jiang-ning，LIU Rong，LI Hong-yan，FAN Ya-wei，LI Jing，DENG Ze-yuan*. Fractional Purification and Structural Analysis of Polysaccharides Extracted from Camellia oleifera Seed Cake[J]. FOOD SCIENCE, 2013, 34(23): 96-102.

参考文献

[1]邢朝宏, 李进伟, 金青哲, 等.我国油茶籽的综合利用[J].粮油食品科技, 2011, (04):13-16.

[2]李文林, 黄凤洪, 王利宾.油茶籽加工和综合利用研究进展[J]. 中国油脂, 2011(11):55-57.

[3]王小蓉.油茶籽湿渣发酵生产蛋白饲料的研究[D]. 湖南农业大学, 2012.

[4]黄振聪, 卢凤来, 李典鹏.茶油精制工艺及应用研究进展[J]. 天然产物研究与开发, 2012(S1):185-189.

[5]丁丽霞, 黎继烈, 钟海雁.发酵法降解油茶粕中茶皂素工艺条件优化[J]. 中国食品学报, 2012(06):71-79.

[6]丁丽霞.油茶粕中茶皂素的降解及其利用研究[D]. 中南林业科技大学, 2012.

[7]Guo L, Zhu Y, Du X F.Compatibility studies on tea polysaccharideamylosewater and tea polysaccharideamylopectinwater[J].CARBOHYDRATE POLYMERS, 2013, 92(1):441-447

[8]He N W, Shi X L, Zhao Y, et al.Inhibitory Effects and Molecular Mechanisms of Selenium-Containing Tea Polysaccharides on Human Breast Cancer MCF-7 Cells[J].JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2013, 61(3):579-588

[9]Wang Y L, Zhao Y, Andrae-Marobela K, et al.Tea polysaccharides as food antioxidants: An old woman's tale?[J].FOOD CHEMISTRY, 2013, 138(2-3):1923-1927

[10]等.Study of Affinity Chromatography Used in the Separation and Purification of the Oil of Tea Seed Polysaccharide I[J].Journal of the Chinese Cereals and Oils Association, 2012, 27(1003-0174（2012）27:112.0.TX;2-E11):34-38

[11]江和源, 陈小强, 寇小红, 等.茶多糖的分级纯化及组成分析[J]. 茶叶科学, 2007(03):248-252.

[12]Wei X L, Mao F F, Cai X, et al.Composition and bioactivity of polysaccharides from tea seeds obtained by water extraction[J].INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2011, 49(4):587-590

[13]陈力, 邓泽元, 胡蒋宁, 等.高速逆流色谱分离制备茶粕中茶皂素单体[J]. 食品科学, 2010(20):127-131.

[14]张丽美, 杨婷婷, 胡蒋宁, 等.超声波辅助提取茶粕多糖及其抗氧化活性研究[J]. 食品科学.

[15]郭金龙, 陈有君, 孙国琴, 等.苯酚-硫酸法测定杏鲍菇多糖方法的研究[J]. 食品科学, 2008(12):555-558.

[16]何念武, 杨兴斌, 田灵敏, 等.黄瓜多糖的体外抗氧化活性[J]. 食品科学, 2011(19):70-74.

[17]宿玮, 常耀光, 薛长湖, 等.海地瓜多糖中蛋白含量测定方法比较[J]. 食品科学, 2011(02):201-204.

[18]Luo Qiushui, Shangguan Xinchen, Jiang Yan, 等.Antioxidant Activity of Glycoprotein from the Purple Sweet Potato[J]. Acta Agriculturae Universitatis Jiangxiensis, 2012, 34:809-813.

[19]Shao Hai, Gong Gangming, Guan Shimin.Study on Extraction and Burification of Glycoprotein from Chinese Yam[J]. Natural Product R & D, 2010, 22:261-263.

[20]Ishihara S, Nakauma M, Funami T, et al.Viscoelastic and fragmentation characters of model bolus from polysaccharide gels after instrumental mastication[J].FOOD HYDROCOLLOIDS, 2011, 25(5):1210-1218

[21]Lin Huajuan, Tian Xiaochun, Qin Xiaoming, 等.Structural Characteristics of Purified Polysaccharide from Camellia chrysantha (Hu) Tuyama[J]. Food Science, 2013, 34:141-146.

[22]毛芳芳.茶源多糖理化性质，结构和活性的研究[D]. 上海师范大学, 2012.

[23]Chen X Q, Lin Z, Ye Y, et al.Suppression of diabetes in non-obese diabetic (NOD) mice by oral administration of water-soluble and alkali-soluble polysaccharide conjugates prepared from green tea[J].CARBOHYDRATE POLYMERS, 2010, 82(1):28-33

[24]Habibi Y, Mahrouz M, Vignon M R.Isolation and structural characterization of protopectin from the skin of Opuntia ficus-indica prickly pear fruits[J].CARBOHYDRATE POLYMERS, 2005, 60(2):205-213

油茶粕多糖的分级纯化及结构研究

Fractional Purification and Structural Analysis of Polysaccharides Extracted from Camellia oleifera Seed Cake

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