猪肉中单增李斯特菌与假单胞菌的竞争生长预测模型建立

doi:10.7506/spkx1002-6630-201323052

食品科学 ›› 2013, Vol. 34 ›› Issue (23): 256-260.doi: 10.7506/spkx1002-6630-201323052

猪肉中单增李斯特菌与假单胞菌的竞争生长预测模型建立

陈尔凝，白羽，马凯，刘清珺，武会娟，高丽娟*

北京市理化分析测试中心，北京 100089

收稿日期:2012-11-13 修回日期:2013-10-24 出版日期:2013-12-15 发布日期:2014-01-03
通讯作者: 高丽娟 E-mail:chen_erning@hotmail.com
基金资助:
北京市科技新星计划项目(2008B31)

Predictive Modeling of the Competitive Growth of Listeria monocytogenes and Pseudomonads spp. in Pork

CHEN Er-ning，BAI Yu，MA Kai，LIU Qing-jun，WU Hui-juan，GAO Li-juan*

Beijing Centre for Physical and Chemical Analysis, Beijing 100089, China

Received:2012-11-13 Revised:2013-10-24 Online:2013-12-15 Published:2014-01-03
Contact: GAO Li-juan E-mail:chen_erning@hotmail.com

摘要/Abstract

摘要：

为预测鲜猪肉的货架期，将Baranyi模型与改良Lotka-Volterra结合，建立猪肉中单增李斯特菌(Listeria monocytogenes)与假单胞菌(Pseudomonads spp.)的竞争生长预测模型。由改良Lotka-Volterra方程计算稳态拮抗系数(α)。结果表明：在低温下单增李斯特菌对假单胞菌有一定抑制作用(α＞0)，且抑制效果在4℃最强；假单胞菌对单增李斯特菌无明显抑制作用。采用ComBase数据库中猪肉中单增李斯特菌的生长数据，计算模型的偏差因子和准确因子，对模型进行数学检验，4℃时偏差因子为1.207表明模型是失败-安全的，准确因子为1.362表明模型预测结果较准确。

关键词: 猪肉, 单增李斯特菌, 假单胞菌, 竞争生长模型, 混合生长

Abstract:

To predict the shelf-life of pork, Baranyi model and Lotka-Volterra model were used to establish a model for the
competitive growth of Listeria monocytogenes and Pseudomonads spp. in pork. Interaction coefficients (α) were calculated
from the steady-state equation of a modified Lotka-Volterra model. The results indicated that at lower temperature,
L. monocytogenes could inhibit the growth of Pseudomonads spp. (α ＞ 0), especially at 4 ℃, which represents the strongest
inhibitory effect, while Pseudomonads spp. showed no significant inhibitory effect on L. monocytogenes. To validate the
model, the L. monocytogenes growth data of in pork acquired from the ComBase database were used to calculate bias and
accuracy factors. At 4 ℃, the bias factor was 1.207, indicating the model to be fail-safe, and the accuracy factor was 1.362,
suggesting good prediction accuracy.

Key words: pork, Listeria monocytogenes, Pseudomonads spp., competitive growth model, mixed growth

中图分类号:

TS201.3

陈尔凝，白羽，马凯，刘清珺，武会娟，高丽娟*. 猪肉中单增李斯特菌与假单胞菌的竞争生长预测模型建立[J]. 食品科学, 2013, 34(23): 256-260.

CHEN Er-ning，BAI Yu，MA Kai，LIU Qing-jun，WU Hui-juan，GAO Li-juan*. Predictive Modeling of the Competitive Growth of Listeria monocytogenes and Pseudomonads spp. in Pork[J]. FOOD SCIENCE, 2013, 34(23): 256-260.

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猪肉中单增李斯特菌与假单胞菌的竞争生长预测模型建立

Predictive Modeling of the Competitive Growth of Listeria monocytogenes and Pseudomonads spp. in Pork

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