食品科学

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水酶法提取番木瓜籽油工艺及其氧化稳定性分析

叶丽红,王标诗*,程漪婷,黄日凤,关清好,江 敏,金 蓓,杜建中   

  1. 岭南师范学院化学科学与技术学院,广东 湛江 524048
  • 出版日期:2014-08-25 发布日期:2014-08-25
  • 通讯作者: 王标诗
  • 基金资助:

    广东省高校优秀青年创新人才培养计划项目(2012LYM_0091);2013年广东省大学生创新创业训练计划项目

Aqueous Enzymatic Extraction and Oxidative Stability of Papaya Seed Oil

YE Li-hong, WANG Biao-shi*, CHENG Yi-ting, HUANG Ri-feng, GUAN Qing-hao, JIANG Min, JIN Bei, DU Jian-zhong   

  1. School of Chemistry Science and Technology, Lingnan Normal University, Zhanjiang 524048, China
  • Online:2014-08-25 Published:2014-08-25
  • Contact: WANG Biao-shi

摘要:

以番木瓜籽为原料,通过单因素试验和正交试验研究不同酶种类、酶解时间、料液比、酶添加量、酶解温度等因素对番木瓜籽油提取率的影响,确定番木瓜籽油提取的最佳工艺条件,并以番木瓜籽油过氧化值为评价指标,考察温度、光照、抗氧化剂对番木瓜籽油氧化稳定性的影响。结果表明,番木瓜籽油的最佳提取条件为:选用中性蛋白酶,酶添加量2.5%、酶解时间5 h、料液比1∶7、酶解温度45 ℃。在此条件下,番木瓜籽油的提取率为85.73%。温度、光照、氧气均会引起贮藏过程中番木瓜籽油过氧化值的升高。添加抗氧化剂可明显提高番木瓜籽油的氧化稳定性,其中叔丁基对苯二酚的抗氧化效果最好。

关键词: 水酶法, 番木瓜籽油, 提取, 氧化稳定性

Abstract:

This study was aimed to establish the optimum conditions for the aqueous enzymatic extraction of papaya seed
oil by investigating the effects of enzymes, hydrolysis time, substrate to water ratio, enzyme dosage and temperature on oil
yield by using single-factor and orthogonal array designs. Meanwhile, the effects of temperature, light and antioxidants on
the oxidative stability of papaya seed oil as reflected by its peroxide value were also analyzed. The results showed that the
optimum parameters for extracting papaya seed oil were found to be 5 h of hydrolysis using neutral protease at a dosage of
2.5% with a substrate to water ratio of 1:7 (g/mL) at 45 ℃. Under the optimized conditions, the extraction yield of papaya
seeds oil was 85.73%. Temperature, light and oxygen caused an increase in peroxide value during storage of papaya seed oil
and TBHQ had good antioxidant effect on the oil.

Key words: aqueous enzymatic method, papaya seed oil, extraction, oxidative stability

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