食品科学

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常压室温等离子体诱变技术选育高产Monacolin K紫色红曲霉突变株

祁田甜1,张 婵1,2,*,胡济美3,郎天丹2,王成涛1,2,*,赵吉兴4   

  1. 1.北京工商大学 北京市食品添加剂工程技术研究中心,北京 100048;
    2.北京工商大学 食品质量与安全北京实验室,北京 100048;3.中国国际工程咨询公司,北京 100048;
    4.山东中惠食品有限公司,山东 滨州 251706
  • 出版日期:2015-05-15 发布日期:2015-05-11

Monascus purpureus Mutant Strains with High Monacolin K Yield Obtained by Atmospheric and Room Temperature Plasmas

QI Tiantian1, ZHANG Chan1,2,*, HU Jimei3, LANG Tiandan2, WANG Chengtao1,2,*, ZHAO Jixing4   

  1. 1. Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University,
    Beijing 100048, China; 2. Beijing Laboratory of Food Quality and Safety, Beijing Technology and Business University,
    Beijing 100048, China; 3. China International Engineering Consulting Corporation, Beijing 100048, China;
    4. Shandong Zhonghui Food Co. Ltd., Binzhou 251706, China
  • Online:2015-05-15 Published:2015-05-11

摘要:

采用常压室温等离子体(atmospheric and room temperature plasma,ARTP)射流诱变技术处理紫色红曲霉菌株M-1,选育高产Monacolin K突变株,为Monacolin K的发酵生产提供优良菌株。确定等离子体处理条件,采用稀释平板培养法挑选突变株,高效液相色谱法分析突变株发酵液的Monacolin K产量,借助扫描电子显微镜观察诱变处理前后菌体微结构特征。结果表明:ARTP对紫色红曲霉菌株具有较强的致死和致突变效应,ARTP处理30 s紫色红曲霉菌株诱变致死率达到84%,处理90 s时其致死率约为92.6%,可获得较高的正突变率(23.8%);筛选得到突变株23的Monacolin K产量达到428.14 mg/L,较初始菌株M-1提高了111%。ARTP作用于紫色红曲霉,可引起菌株形态学特征发生改变,突变株的菌落色泽、菌丝体和孢子形态等特征均有一定变化;ARTP产生的活性粒子可透过细胞膜作用于DNA物质,引起DNA发生多样性损伤、不完全修复突变,形成遗传稳定的突变株。

关键词: 常压室温等离子体, 紫色红曲霉, Monacolin K, 突变菌株, 扫描电子显微镜

Abstract:

Atmospheric and room temperature plasma (ARTP) jet was used to induce Monascus purpureus M-1, in order
to obtain mutant strains with enhanced ability to produce monacolin K. The optimal treatment conditions were determined,
and dilution plate culture method was used to screen mutant strains. HPLC was used to analyze monacolin K production,
and scanning electron microscopy was used to observe microstructure characteristics of the strain M-1 before and after
mutagenesis. Results showed that ARTP had a stronger lethal effect on Monascus purpureus M-1, and the fatality rate of
Monascus purpureus reached 84% after 30 s of ARTP treatment. When treated for 90 s, the fatality rate was about 92.6%,
with higher mutation rate (23.8%), and monacolin K yield of the mutant 23 was 428.14 mg/L, which was increased by 111%
when compared with that of the initial strain M-1. ARTP resulted in morphological changes of Monascus purpureus in terms of
colony color, mycelium and spore morphological characteristics. Active particles produced by ARTP could lead to DNA damage
and even non-repairable mutations through penetrating the cell membrane to form the genetic stability of the mutant strains.

Key words: atmospheric and room temperature plasma (ARTP), Monascus purpureus, monacolin K, mutant strains, scanning electron microscope

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