食品科学
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崔月倩,王菁蕊,王艳萍*
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CUI Yueqian, WANG Jingrui, WANG Yanping*
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摘要:
近年来随着乳酸菌的功能特性和应用研究的深入,利用分子手段研究乳酸菌的功能基因已经成为最新研究热点。研究者构建众多不同特性的基因表达载体,本文从载体使用的安全性角度对已经报道的载体进行总结,并介绍各类基因表达载体在发酵产胞外多糖、酶制剂、疫苗制备等方面的应用。
关键词: 乳酸菌, 非食品级基因表达载体, 食品级基因表达载体, 应用
Abstract:
In recent years, with the further functional study on the characteristics and applications of lactic acid bacteria, molecular biology of lactic acid bacteria is also of concern. Cloning and functional expression of lactic acid bacteria using molecular tools have become the latest hotspot. Researchers have found or built many gene expression vectors with different characteristics which lay the foundation for gene cloning and expression of lactic acid bacteria. Based on the characteristics of lactic acid bacteria, this paper summarizes the vectors which have been reported from the perspective of safety. And we also review the applications of gene expression vectors for the production of polysaccharides, enzyme and vaccine preparation, etc.
Key words: lactic acid bacteria, non-food-grade genetic expression vector, food-grade genetic expression vector, application
中图分类号:
Q782
崔月倩,王菁蕊,王艳萍*. 乳酸菌基因表达载体及其应用研究进展[J]. 食品科学, doi: 10.7506/spkx1002-6630-201509042.
CUI Yueqian, WANG Jingrui, WANG Yanping*. A Review of Research on Lactic Acid Bacteria Vectors for Gene Expression and Their Applications[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201509042.
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链接本文: https://www.spkx.net.cn/CN/10.7506/spkx1002-6630-201509042
https://www.spkx.net.cn/CN/Y2015/V36/I9/224