黑木耳多糖体外和体内降血糖功能

doi:10.7506/spkx1002-6630-201521041

食品科学

黑木耳多糖体外和体内降血糖功能

尹红力1,2，赵鑫1，佟丽丽3，王振宇4

1.东北林业大学林学院，黑龙江哈尔滨 150040；2.中国农业科学院农产品加工研究所主食加工技术研究院，黑龙江
哈尔滨 151900；3.牡丹江医学院红旗医院，黑龙江牡丹江 157011；4.哈尔滨工业大学化工学院，黑龙江哈尔滨 150090

出版日期:2015-11-15 发布日期:2015-12-03

In Vitro and in Vivo Anti-Hyperglycemic Effects of Polysaccharides from Auricularia auricular

YIN Hongli1,2, ZHAO Xin1, TONG Lili3, WANG Zhenyu4

1. School of Forestry, Northeast Forestry University, Harbin 150040, China; 2. Institute of Staple Food Processing Technology,
Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Harbin 151900, China;
3. Hongqi Hospital of Mudanjiang Medical University, Mudanjiang 157011, China;
4. School of Chemical Engineering and Technology, Harbin Institute of Technology, Harbin 150090, China

Online:2015-11-15 Published:2015-12-03

摘要/Abstract

摘要：

目的：探讨黑木耳多糖体内和体外降血糖功能。方法：通过建立体外α-葡萄糖苷酶抑制剂微孔筛选模型，研究黑木耳多糖不同提取物及黑木耳酸性多糖不同醇沉片段对α-葡萄糖苷酶的体外抑制作用。以四氧嘧啶诱导建立糖尿病小鼠模型，通过黑木耳酸性多糖的治疗，比较治疗前后小鼠体质量及空腹血糖值的变化、测定治疗后小鼠体内糖代谢关键酶——己糖激酶和琥珀酸脱氢酶的活性，研究黑木耳酸性多糖对四氧嘧啶致糖尿病小鼠的降血糖作用。结果：体外降血糖效果表明，黑木耳多糖有抑制α-葡萄糖苷酶的作用，3 种不同的黑木耳多糖样品中，黑木耳酸性多糖抑制α-葡萄糖苷酶的活性最强，黑木耳中性多糖次之，黑木耳碱性多糖最弱。黑木耳酸性多糖不同醇沉片段中，80%醇沉片段抑制α-葡萄糖苷酶的活性最强。体内降血糖效果表明，黑木耳酸性多糖80%醇沉片段可减缓糖尿病小鼠体质量的负增长，缓解己糖激酶、琥珀酸脱氢酶活性的降低。本实验结果表明，黑木耳酸性多糖具有明显的降血糖作用。

关键词: 黑木耳多糖, 黑木耳酸性多糖, &alpha, -葡萄糖苷酶, 糖尿病

Abstract:

Objective: To explore the hypoglycemic effects of Auricularia auricular polysaccharides in vitro and in
vivo. Methods: The inhibitory effects of acidic, neutral and alkaline polysaccharides from Auricularia auricular as
well as ethanol-precipitated fractions of acidic polysaccharides on α-glucosidase activity were investigated by in vitro
microwell plate-based screening method. The purified polysaccharide was used to treat rat models of diabetes induced by
alloxan. The changes in body weight, fasting blood glucose (FBG), hexokinase (HK), and succinic dehydrogenase (SDH)
were determined in diabetic rats before and after treatment. Results: Based on the hypoglycemic effect in vitro, Auricularia
auricular polysaccharides had inhibitory effect on α-glucosidase. Auricularia auricular acidic polysaccharides had the
strongest inhibitory activity against α-glucosidase followed by the neutral polysaccharides, and the alkaline polysaccharides
were the weakest α-glucosidase inhibitor. The inhibitory activity of 80% alcohol-precipitated fraction was stronger than
that of other alcohol concentrations. According to in vivo hypoglycemic results, acidic polysaccharides from 80% alcohol
precipitation fraction could significantly attenuate the negative growth of body weights in diabetic rats, and significantly
increase HK and SDH. Therefore, Auricularia auricular acidic polysaccharides have significant hypoglycemic effect.

Key words: Auricularia auricular polysaccharides, Auricularia auricular acidic polysaccharides, α-glucosidase, diabetes

中图分类号:

TS201.4

尹红力，赵鑫，佟丽丽，王振宇. 黑木耳多糖体外和体内降血糖功能[J]. 食品科学, doi: 10.7506/spkx1002-6630-201521041.

YIN Hongli, ZHAO Xin, TONG Lili, WANG Zhenyu. In Vitro and in Vivo Anti-Hyperglycemic Effects of Polysaccharides from Auricularia auricular[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201521041.

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黑木耳多糖体外和体内降血糖功能

In Vitro and in Vivo Anti-Hyperglycemic Effects of Polysaccharides from Auricularia auricular

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