食品科学

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采用牛血清白蛋白/果糖体系研究荧光性AGEs生成的动力学

胡 静,李巨秀*   

  1. 西北农林科技大学食品科学与工程学院,陕西 杨陵 712100
  • 出版日期:2017-01-15 发布日期:2017-01-16

Formation Kinetics of Fluorescent AGEs in Bovine Serum Albumin/Fructose System

HU Jing, LI Juxiu*   

  1. College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China
  • Online:2017-01-15 Published:2017-01-16

摘要: 为分析荧光性晚期糖基化末端产物(advanced glycation end products,AGEs)生成的动力学,采用牛血清白蛋白(bovine serum albumin,BSA)/果糖模拟反应体系,荧光光谱法测定荧光强度来衡量荧光性AGEs生成量,分别考察了不同BSA质量浓度、果糖浓度、温度及pH值对荧光性AGEs生成量的影响,探究荧光性AGEs生成和底物消耗的反应动力学。结果表明:随着BSA质量浓度、果糖浓度、温度及pH值的增加,荧光性AGEs生成量均呈现增加的趋势。体系反应24 min后,果糖和氨基酸浓度分别减少了1.25×10-2、1.92×10-4 mol/L,果糖消耗量要高于氨基酸消耗量。在此基础上,运用最小二乘法拟合,得出在各反应条件下,荧光性AGEs的生成符合0级反应动力学,最大速率常数为31.57 AU/min,反应活化能为70.58 kJ/mol;底物消耗符合1级反应动力学。

关键词: 晚期糖基化末端产物(AGEs), 牛血清白蛋白/果糖体系, 动力学, 反应级数

Abstract: In order to analyze the formation kinetics of fluorescent advanced glycation end products (AGEs), this work
addressed the effects of bovine serum albumin (BSA) concentration, fructose concentration, temperature, and pH on the
formation of fluorescent AGEs in a simulated processing system consisting of BSA and glucose. Fluorescence intensity was
used to measure the fluorescent AGEs content. The kinetics of substrate depletion was also investigated. The results showed
that the fluorescent AGEs content increased with increasing BSA concentration, fructose concentration, temperature and pH.
After 24 min reaction, fructose and amino acid concentrations decreased by 1.25 × 10-2 and 1.92 × 10-4 mol/L, respectively,
indicating a larger decrease in fructose concentration. The formation of fluorescent AGEs followed zero-order kinetics,
with a maximum rate constant of 31.57 AU/min and an activation energy of 70.58 kJ/mol, and substrate depletion followed
first-order kinetics.

Key words: advanced glycation end products (AGEs), bovine serum albumin/fructose system, kinetics, reaction order

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