食品科学 ›› 2019, Vol. 40 ›› Issue (3): 348-355.doi: 10.7506/spkx1002-6630-20171204-049

• 专题论述 • 上一篇    

高通量测序技术在酿酒微生物多样性研究中的应用

吴?成,王春晓*,王晓丹,周鸿翔,黄永光,邱树毅*   

  1. (贵州大学酿酒与食品工程学院,贵州省发酵工程与生物制药重点实验室,贵州?贵阳 550025)
  • 出版日期:2019-02-15 发布日期:2019-03-05
  • 基金资助:
    贵州省科技攻关项目(黔科合GZ字[2014]2012);贵州省科技重大专项(黔科合科技重大专项字[2015]6012); 贵州省科技支撑计划项目(黔科合支撑[2016]2340;黔科合支撑[2016]2557-1); 贵州大学引进人才科研项目(贵大人基合字[2017]44号)

A Review of the Application of High-Throughput Sequencing Technology in Analysis of the Diversity of Microbial Communities Involved in the Fermentation of Alcoholic Beverages

WU Cheng, WANG Chunxiao*, WANG Xiaodan, ZHOU Hongxiang, HUANG Yongguang, QIU Shuyi*   

  1. (Province Key Laboratory of Fermentation Engineering and Biopharmacy, School of Liquor and Food Engineering, Guizhou University, Guiyang 550025, China)
  • Online:2019-02-15 Published:2019-03-05

摘要: 高通量测序技术是分析研究酿酒微生物多样性的重要手段,与其他方法相比,具有测序方法简单、通量高、速度快和准确度较高等特点。本文在阐述高通量测序技术特点和测序平台的基础上,着重介绍其在酿酒微生物多样性研究领域中常用的数据分析方法和应用现状,旨在为酿酒微生物多样性研究者提供较为系统、全面的关于高通量测序技术的使用策略。

关键词: 高通量测序平台, 主成分分析, 相对丰度热图, 白酒, 葡萄酒

Abstract: High-throughput sequencing technology is one of the important methods used to analyze the diversity of microbial communities involved in the fermentation of alcoholic beverages. Compared with other technologies, this has the advantages of simplicity, high throughput, high sequencing speed and good accuracy. In this article, we describe the characteristics of high-throughput sequencing technology and commonly used high-throughput sequencing platforms. Our focus is on data analysis methods commonly used for the application of high-throughput sequencing technology to analyze the diversity of microbial communities involved in the fermentation of alcoholic beverages and on the current status of the application of high-throughput sequencing technology. The aim of this review is to provide useful information about the use of high-throughput sequencing technology for researchers?engaged in this field.

Key words: high-throughput sequencing platforms, principal component analysis, relative abundance heatmap, liquor, wine

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