食品科学 ›› 2017, Vol. 38 ›› Issue (22): 211-215.doi: 10.7506/spkx1002-6630-201722032

• 成分分析 • 上一篇    下一篇

基于氧化氮掺杂石墨烯修饰碳糊电极高灵敏测定辣椒素

牙禹,蒋翠文,李焘,唐莉,宁德娇,闫飞燕,谢丽萍   

  1. (广西壮族自治区农业科学院农产品质量安全与检测技术研究所,农业部甘蔗品质监督检验测试中心(南宁),广西?南宁 530007)
  • 出版日期:2017-11-25 发布日期:2017-11-03
  • 基金资助:
    广西自然科学基金青年基金项目(2015GXNSFBA139037);广西农科院优势学科团队项目(桂农科2015YT94)

Highly Sensitive Determination of Capsaicin Based on Nitrogen-Doped Graphene Oxide Modified Carbon Paste Electrode

YA Yu, JIANG Cuiwen, LI Tao, TANG Li, NING Dejiao, YAN Feiyan, XIE Liping   

  1. (Quality Inspection and Test Center for Sugarcane and Its Product (Nanning), Ministry of Agriculture, Agro-Products Quality Safety and Testing Technology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China)
  • Online:2017-11-25 Published:2017-11-03

摘要: 采用一步水热合成法制备氧化氮掺杂石墨烯(nitrogen-doped graphene oxide,NGO),采用X射线光电子能谱,透射电子显微镜对其进行表征。采用滴涂法制备了氧化氮掺杂石墨烯修饰碳糊电极(nitrogen-doped graphene oxide/carbon paste electrode,NGO/CPE),研究辣椒素在NGO/CPE上的电化学行为,发现与裸碳糊电极相比,NGO/CPE能显著增加辣椒素的响应信号。优化了NGO质量浓度、富集时间、介质pH值等实验参数,利用差分脉冲伏安法对辣椒素进行测定,辣椒素氧化峰电流与其质量浓度在5.0~1?200?μg/L范围内呈良好线性关系,检出限为2.0?μg/L(RSN=3)。将本方法用于辣椒粉样品中辣椒素含量检测,结果与比色法基本一致。

关键词: 氧化氮掺杂石墨烯, 辣椒素, 修饰电极, 电分析, 差分脉冲伏安法

Abstract: Nitrogen-doped graphene oxide (NGO) was synthesized by a one-step hydrothermal method and characterized by X-ray photoelectron spectroscopy (XPS) and transmission electron microscopy (TEM). A NGO modified carbon paste electrode (NGO/CPE) was prepared, and the electrochemical behaviors of capsaicin at NGO/CPE were studied. Experimental results showed that the voltammetric response of capsaicin at NGO/CPE was greatly improved when compared with bare carbon paste electrode (CPE). Some parameters such as NGO concentration, accumulation time and pH were optimized. Capsaicin was determined by using differential pulse voltammetry. Under optimal conditions, the peak current was linearly proportional to capsaicin concentration in the range of 5.0 to 1 200 μg/L, with a limit of detection (LOD) of 2.0 μg/L (RSN = 3). The proposed method was applied for the determination of capsaicin in hot pepper samples, and the results well agreed with those obtained by colorimetry.

Key words: nitrogen-doped graphene oxide, capsaicin, modified electrode, electroanalysis, differential pulse voltammetry

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