桑葚浓缩汁对D-半乳糖诱导氧化损伤大鼠肾脏的保护作用

doi:10.7506/spkx1002-6630-201723029

食品科学 ›› 2017, Vol. 38 ›› Issue (23): 184-190.doi: 10.7506/spkx1002-6630-201723029

桑葚浓缩汁对D-半乳糖诱导氧化损伤大鼠肾脏的保护作用

黄磊1，彭新颜1,*，于海洋2，李华敏1，毛慧敏1，张辉1，邱静茹1

1.鲁东大学食品工程学院，山东烟台 264025；2.山东商务职业学院食品工程系，山东烟台 264670

出版日期:2017-12-15 发布日期:2017-12-07
基金资助:
国家自然科学基金青年科学基金项目（31401491）；城新创新奖学金项目；大学生创新创业训练计划项目（7422）

Protective Effect of Mulberry Juice Concentrate on D-Galactose-Induced Oxidative Stress in Kidney of Rats

HUANG Lei1, PENG Xinyan1,*, YU Haiyang2, LI Huamin1, MAO Huimin1, ZHANG Hui1, QIU Jingru1

1. College of Food Engineering, Ludong University, Yantai 264025, China; 2. Department of Food Engineering, Shandong Business Institute, Yantai 264670, China

Online:2017-12-15 Published:2017-12-07

摘要/Abstract

摘要： 研究桑葚浓缩汁对氧化损伤大鼠肾脏的保护作用。采用D-半乳糖（D-galactose，D-Gal）建立衰老模型，将实验大鼠随机分为6 组：空白对照组、阴性对照组（D-Gal模型）、阳性对照组（D-Gal＋VE）、桑葚浓缩汁低、中、高剂量组。通过检测大鼠肾脏组织匀浆中超氧化物歧化酶（superoxide dismutase，SOD）、过氧化氢酶（catalase，CAT）和谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）活力、丙二醛（malondialdehyde， MDA）含量，测定大鼠血清一氧化氮（nitric oxide，NO）、血尿素氮（blood urea nitrogen，BUN）和血清肌酐（serum creatinine，Scr）浓度、总抗氧化能力（total antioxidant capacity，T-AOC）及B淋巴细胞瘤-2相关X蛋白（B-cell lymphoma-2-associated X protein，Bax）基因的表达，并通过病理组织学切片评价桑葚浓缩汁对氧化损伤蛋白的影响。结果表明：与阴性对照组相比，桑葚浓缩汁中、高剂量组可以显著提高肾组织SOD、GSH-Px、 CAT活力和T-AOC（P＜0.05）；降低MDA、BUN及Scr浓度；抑制NO浓度升高（P＜0.05），并成一定量效依赖关系；Bax表达降低；其中桑葚浓缩汁高剂量效果最好，GSH-Px活力为150.33 U、Scr浓度为34.54 μmol/L、 BUN浓度为8.96 mmol/L，均达到了阳性对照组水平（P＞0.05）；NO浓度为29.78 μmol/L，达到空白对照组水平（P＞0.05）。病理学组织切片显示，与阴性对照组相比，桑葚处理组症状明显减轻，尤其中、高剂量组，极少量肾小管上皮细胞胞浆疏松、淡染，无明显的充血、出血。综上所述，桑葚浓缩汁对D-Gal诱导氧化损伤大鼠肾脏有较好的保护作用。

关键词: 桑葚浓缩汁, D-半乳糖, 氧化损伤, 肾脏保护作用

Abstract: To study the protective effect of mulberry juice concentrate on oxidative injury in the kidney of rats, an aging rat model was established by injection of D-galactose (D-Gal) and the experimental rats were divided into six groups, including normal control, negative control, positive control group (D-Gal + VE), D-Gal + mulberry juice concentrate low-, middle- and high-dose groups. Superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities and malondialdehyde (MDA) content in liver homogenate, nitric oxide (NO), urea nitrogen (BUN), creatinine (Scr) and total antioxidant capacity (T-AOC) in serum, and the expression of B-cell lymphoma-2-associated X protein (Bax) gene in kidney were measured. Besides, hematoxylin-eosin stained kidney sections were histopathologically examined. The results showed that compared with the negative control group, mulberry juice concentrate at middle and high doses could significantly increase the activities of SOD, GSH-Px, CAT and T-AOC in renal tissue (P < 0.05), decrease MDA level in renal tissue, serum BUN and Scr levels and repress the increase in serum NO (P < 0.05) in a dose-dependent manner. In addition, the expression of Bax was significantly reduced (P < 0.05), and the best result was observed at high dose, where GSH-Px activity was 150.33 U and serum Scr and BUN were 34.54 μmol/L and 8.96 mmol/L, respectively, which were close to the levels of the positive control group (P > 0.05), and serum NO was 29.78 μmol/L, which was similar to that of the blank control group (P > 0.05). Histopathological examination showed that compared with the model group, the symptoms of kidney injury in the treatment groups were obviously alleviated, especially in the middle- and high-dose groups, where few renal tubular epithelial cells were loose and pale, without obvious congestion or bleeding. In summary, mulberry juice concentrate has a good protective effect on D-Gal-induced oxidative stress in the kidney of rats.

Key words: mulberry juice concentrate, D-galactose, oxidative damage, renal protection

中图分类号:

TS255.2

黄磊，彭新颜，于海洋，李华敏，毛慧敏，张辉，邱静茹. 桑葚浓缩汁对D-半乳糖诱导氧化损伤大鼠肾脏的保护作用[J]. 食品科学, 2017, 38(23): 184-190.

HUANG Lei, PENG Xinyan, YU Haiyang, LI Huamin, MAO Huimin, ZHANG Hui, QIU Jingru. Protective Effect of Mulberry Juice Concentrate on D-Galactose-Induced Oxidative Stress in Kidney of Rats[J]. FOOD SCIENCE, 2017, 38(23): 184-190.

[1]	徐颖，王斌，姜启兴，夏文水，许艳顺. 壳寡糖对酒精诱导的大鼠肠道损伤的干预作用[J]. 食品科学, 2021, 42(3): 158-164.
[2]	张羽，汪芳，翁泽斌，包伊凡，宋海昭，沈新春. 麦胚清蛋白抗氧化肽的筛选及对细胞氧化损伤的保护作用[J]. 食品科学, 2021, 42(17): 10-18.
[3]	梁曾恩妮，汪秋安，张菊华，苏东林，付复华，李高阳，刘伟，单杨. 圣草次苷的合成及其对过氧化氢诱导人脐静脉内皮细胞氧化损伤的保护作用[J]. 食品科学, 2021, 42(15): 159-166.
[4]	翟兵中，张丽婧，刘臻，陈建国，胡志航，梅松，胡文力，楼敏涵，王茵，曲雪峰. 姜黄素调节Na/K-ATPase/Src信号通路保护LLC-PK1细胞缺氧复氧损伤[J]. 食品科学, 2021, 42(15): 136-142.
[5]	薛宏坤，谭佳琪，李倩，唐劲天. 矢车菊素-3-O-葡萄糖苷保护RAW264.7细胞免受过氧化氢诱导的氧化损伤[J]. 食品科学, 2021, 42(13): 103-113.
[6]	于兰兰，刘伟，周雅琳，李雍，秦勇，李睿珺，许雅君. 小麦低聚肽对急性酒精中毒小鼠抗氧化功能的影响[J]. 食品科学, 2020, 41(7): 159-163.
[7]	冯晴霞，闫宇宁，杨峄，周嘉宁，李乐斌，卢学春，安丽萍. 姬松茸多肽提取物对D-半乳糖致衰老模型小鼠的保护作用[J]. 食品科学, 2020, 41(7): 164-170.
[8]	杨忠敏，王祖文，黄先智，丁晓雯. 桑叶生物碱对D-半乳糖诱导小鼠生物大分子氧化损伤的改善作用及机理[J]. 食品科学, 2020, 41(5): 135-142.
[9]	郑义，张健，曹永强，赵笑，余志坚，陈超，杨贞耐. 产β-D-半乳糖苷酶马克斯克鲁维酵母的分离鉴定及其产酶特性[J]. 食品科学, 2020, 41(2): 158-165.
[10]	李桐，吴思琪，曹鑫，宋静颐，张红星，谢远红，金君华. 具抗氧化功能益生菌菌株筛选及其对丙烯酰胺诱导肠上皮细胞氧化损伤的保护作用[J]. 食品科学, 2020, 41(2): 173-180.
[11]	杨忠敏，沈以红，王祖文，黄先智，丁晓雯. 桑叶生物碱对D-半乳糖诱导氧化损伤模型小鼠肾脏的改善作用[J]. 食品科学, 2020, 41(19): 198-203.
[12]	杨忠敏，沈以红，黄先智，王祖文，丁晓雯. 桑叶生物碱粗提物对D-半乳糖诱导的小鼠蛋白氧化损伤的改善作用[J]. 食品科学, 2020, 41(17): 182-187.
[13]	李浩铭, 黄永杰, 王永丽, 李大鹏, 李锋. 姜黄素及其代谢修饰产物对PC12细胞氧化损伤的保护作用[J]. 食品科学, 2020, 41(15): 208-215.
[14]	何传波, 邓婷, 魏好程, 吴国宏, 上官宇晨, 熊何健. 仙草多糖对细胞氧化损伤的保护作用[J]. 食品科学, 2020, 41(13): 160-168.
[15]	黄城龙，朱利娟，易想炼，黄琳，欧朝萍，马玉容，邬静，李荣芳，易金娥. 桦木酸对T-2毒素致小鼠肠道氧化损伤的保护作用[J]. 食品科学, 2020, 41(11): 194-200.

桑葚浓缩汁对D-半乳糖诱导氧化损伤大鼠肾脏的保护作用

Protective Effect of Mulberry Juice Concentrate on D-Galactose-Induced Oxidative Stress in Kidney of Rats

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价