食品科学 ›› 2019, Vol. 40 ›› Issue (16): 308-313.doi: 10.7506/spkx1002-6630-20180611-168

• 安全检测 • 上一篇    下一篇

霉菌检测中封闭式培养方法的建立和应用

韩 涛,杨雪妮,张 芳   

  1. 1.福建省产品质量检验研究院,福建 福州 350002;2.福建中医药大学药学院,福建 福州 350108
  • 出版日期:2019-08-25 发布日期:2019-08-26
  • 基金资助:
    福建省质量技术监督局科技项目(FJQI2017013);福建省产品质量检验研究院科技项目(KY201721A)

Establishment and Application of a Closed Culture Method for Enumeration of Molds

HAN Tao, YANG Xueni, ZHANG Fang   

  1. 1. Fujian Inspection and Research Institute for Product Quality, Fuzhou 350002, China; 2. College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China
  • Online:2019-08-25 Published:2019-08-26

摘要: 通过微氧胁迫下黑曲霉的生长动态研究,构建霉菌的封闭式培养方法,并用于食品中霉菌的检测,对比分析霉菌封闭式培养方法的可行性和实用性。结果表明,建立的封闭式培养方法与传统敞开式培养方法的计数结果无显著差异。因此,对于食品中霉菌计数,可以采用以空气与培养基的体积比不小于3为参数的封闭式培养方法,代替传统的敞开式培养方法,以解决霉菌孢子扩散和污染的难题。

关键词: 霉菌, 封闭式培养, 食品, 计数, 检测

Abstract: In the present study, we focused on the growth dynamics of Aspergillus niger under-micro oxygen stress to develop a closed culture method for the detection of this mold in foods. A comparative analysis was made to explore the feasibility and practicality of the culture method. The results showed that there was no significant difference between the closed culture and the traditional open culture. Therefore, the closed culture method featuring the parameter of volume ratio between air and medium equal to or more than 3 could be used to determine mold counts in foods in place of the traditional open culture method, so as to overcome the problems of mold spore diffusion and pollution.

Key words: molds, closed culture method, food, enumeration, detection

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