食品科学 ›› 2019, Vol. 40 ›› Issue (15): 78-83.doi: 10.7506/spkx1002-6630-20180613-203

• 基础研究 • 上一篇    下一篇

薰衣草精油微乳体系的相行为、微观结构转变和清除自由基活性

宋晓秋,王丽萍,孙 月,李帅涛,张 芊,叶 琳,杨晓波,王一非   

  1. 上海应用技术大学香料香精技术与工程学院,上海 201418
  • 出版日期:2019-08-15 发布日期:2019-08-26
  • 基金资助:
    上海市高校实验技术队伍建设个人项目(SYyyz18004)

Phase Behavior, Microstructure Transformation and Free Radical Scavenging Activity of Lavender Essential Oil Microemulsion Systems

SONG Xiaoqiu, WANG Liping, SUN Yue, LI Shuaitao, ZHANG Qian, YE Lin, YANG Xiaobo, WANG Yifei   

  1. School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 201418, China
  • Online:2019-08-15 Published:2019-08-26

摘要: 薰衣草精油具有镇静安神、抗氧化等特性,但其疏水性限制了其在食品行业中的应用。本研究通过微乳化技术构建薰衣草精油微乳液运载体系,研究其相行为及微观结构转变对其清除自由基活性的影响。通过三元相图发现,二丙二醇作为助表面活性剂可以把微乳单相区面积扩大至71.4%;通过染色法、电导率的测定,确定微乳体系微观结构转变,水分质量分数分别为0%~30%、40%~50%、60%~90%时,体系相应处于W/O、双连续和O/W的结构类型,且在双连续阶段体系黏度达到峰值。同时,微乳液对1,1-二苯基-2-三硝基苯肼自由基清除率随着水分体积分数增加而提高;2,2’-联氮-双-3-乙基苯并噻唑啉-6-磺酸阳离子自由基清除率在W/O和双连续型阶段先下降,转变为O/W型时回升,并在水分体积分数70%时达到最大值。该多元微乳体系有效实现了薰衣草精油的增溶,且其物理特征和抗氧化性的变化与微观结构密切相关。

关键词: 薰衣草精油, 微乳体系, 相行为, 微观结构转变, 自由基清除能力

Abstract: Recently, plant essential oil is increasingly applied as a bioactive component in the food industry. Lavender essential oil has multiple bioactivities such as sedative, hypnotic and antioxidant effects. However, poor hydrophobic properties limit its application in food products. In the present study, lavender essential oil microemulsion systems were constructed to evaluate their phase behavior and to investigate the influence of microstructure transformation on their free radical scavenging activity. According to the ternary phase diagram, dipropylene glycol acted as a co-surfactant to expand the single-phase area of microemulsions to 71.4%. Based on the results of dye diffusion and conductivity measurement, the dispersion phase was gradually inverted from a W/O stage (0%–30% water content) to a bicontinuous stage (40%–50% water content), and finally an O/W stage (60%–90% water content) along the dilution line. The viscosity peaked at the bicontinuous stage. This study found that the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity was increased with the increase of water content. In addition, 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging capacity was reduced at the W/O and bicontinuous stages, followed by an increase at the O/W stage, peaking at a 70% water content. In conclusion, the systems can effectively improve the solubility of lavender essential oil, and there is a close association of their physical characteristics and antioxidant effect with microstructure transition.

Key words: lavender essential oil, microemulsion, phase behavior, microstructure transformation, free radical scavenging capacity

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