果胶多糖水热法降解及其产物体外抗氧化性评价

doi:10.7506/spkx1002-6630-20180724-295

食品科学 ›› 2019, Vol. 40 ›› Issue (12): 253-259.doi: 10.7506/spkx1002-6630-20180724-295

果胶多糖水热法降解及其产物体外抗氧化性评价

姜美云1,2，唐硕2，王婷2，赖晨欢1,2，范一民1,2，勇强1,2,*

1.南京林业大学林业资源高效加工利用协同创新中心，江苏南京 210037；2.南京林业大学化学工程学院，江苏南京 210037

出版日期:2019-06-25 发布日期:2019-06-28
基金资助:
“十三五”国家重点研发计划重点专项（2016YFD0600803）

Degradation of Pectic Polysaccharide by Hydrothermal Treatment and Antioxidant Activity in Vitro of the Resulting Products

JIANG Meiyun1,2, TANG Shuo2, WANG Ting2, LAI Chenhuan1,2, FAN Yimin1,2, YONG Qiang1,2,*

1. Collaborative Innovation Center for Efficient Utilization of Forestry Resources, Nanjing Forestry University, Nanjing 210037, China; 2. College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China

Online:2019-06-25 Published:2019-06-28

摘要/Abstract

摘要： 采用水热法降解商品果胶多糖，并对其降解产物的抗氧化活性进行评价。结果表明，水热法降解果胶多糖的最优工艺条件为水热处理温度140 ℃、水热处理时间30 min、pH 6；在此条件下，果胶多糖降解产物得率达46.2%。在此基础上，采用乙醇分级沉淀法对果胶多糖水热处理液进行分离，得到3 种不同分子质量范围的果胶多糖降解产物（S1、S2和S3），其重均分子质量分别为13.4、7.5 kDa和5.7 kDa。以商品果胶多糖和3 种降解产物为研究对象，进行抗氧化性评价，结果表明，S1组分对1,1-二苯基-2-苦基肼自由基的清除率达49.8%，是商品果胶的4 倍；S3组分对超氧阴离子自由基的清除率达58.7%，是商品果胶的10 倍。说明水热降解果胶多糖可显著提高其抗氧化活性，为果渣废弃物的高效利用提供理论依据。

关键词: 果胶多糖, 水热法, 降解产物, 乙醇分级分离, 分子质量, 抗氧化活性

Abstract: In this work, hydrothermal treatment was carried out on a commercial pectic polysaccharide, and the antioxidant activities of the degradation products were investigated. The results showed that the optimal degradation conditions were found to be treatment at 140 ℃ and pH 6 for 30 min. Under these conditions, the efficiency of degradation was 46.2%. On this basis, the degradation products were fractionated by ethanol precipitation into three fractions (S1, S2 and S3). The weight average molecular masses of S1, S2 and S3 were 13.4, 7.5 and 5.7 kDa, respectively. The antioxidant activities of the pectic polysaccharide and the three degradation products were evaluated. The results showed that the 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging rate of S1 was 49.8%, which was 4 times as high as that of the pectin, while the superoxide anion scavenging rate of S3 was 58.7%, which was 10 times as high as that of the pectin. These results indicated that the hydrothermal degradation of pectic polysaccharides could significantly improve its antioxidant activity. This study provides a theoretical basis for the efficient utilization of pomace wastes.

Key words: pectic polysaccharide, hydrothermal treatments, degradation products, ethanol fractionation, molecular mass, antioxidant activity

中图分类号:

TS201.1

姜美云，唐硕，王婷，赖晨欢，范一民，勇强. 果胶多糖水热法降解及其产物体外抗氧化性评价[J]. 食品科学, 2019, 40(12): 253-259.

JIANG Meiyun, TANG Shuo, WANG Ting, LAI Chenhuan, FAN Yimin, YONG Qiang. Degradation of Pectic Polysaccharide by Hydrothermal Treatment and Antioxidant Activity in Vitro of the Resulting Products[J]. FOOD SCIENCE, 2019, 40(12): 253-259.

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果胶多糖水热法降解及其产物体外抗氧化性评价

Degradation of Pectic Polysaccharide by Hydrothermal Treatment and Antioxidant Activity in Vitro of the Resulting Products

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