食品科学 ›› 2018, Vol. 39 ›› Issue (8): 224-230.doi: 10.7506/spkx1002-6630-201808035

• 安全检测 • 上一篇    下一篇

基于纳米金的过氧化物模拟酶比色检测乐果

邹小波,石海军,黄晓玮,石吉勇,翟晓东,蒋彩萍,李志华   

  1. (江苏大学食品与生物工程学院,江苏?镇江 212013)
  • 出版日期:2018-04-25 发布日期:2018-04-17
  • 基金资助:
    国家自然科学基金面上项目(31671844;31601543);“十二五”国家科技支撑计划项目(2015BAD17B04); “十三五”国家重点研发计划重点专项(2016YFD0401104);江苏省自然科学基金项目(BK20160506); 中国博士后科学基金项目(2016M590422;2017T100334)

Colorimetric Detection of Rogor Using Gold Nanoparticles as Peroxidase Mimetics

ZOU Xiaobo, SHI Haijun, HUANG Xiaowei, SHI Jiyong, ZHAI Xiaodong, JIANG Caiping, LI Zhihua   

  1. (School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China)
  • Online:2018-04-25 Published:2018-04-17

摘要: 以金纳米粒子(gold nanoparticles,AuNPs)作为过氧化物模拟酶建立一种简单、快速、可视化的有机磷农药检测方法。以金晶种生长法制得平均直径为25?nm表面带正电性球形AuNPs,这种AuNPs具有明显的过氧化物模拟酶的活性;优化反应条件实验得出在pH?4.0、温度35?℃、四甲基联苯胺浓度1?mmol/L和H2O2浓度10?mmol/L时模拟酶具有最强的催化活性;通过比色分析法实现对有机磷农药检测,实验发现乐果在1~80?mmol/L浓度范围与显色抑制率呈良好的线性关系,相关系数R2为0.985,检出限为1.39?mmol/L,在茶叶样本中的加标回收率在91.36%~102.56%之间;AuNPs在室温条件下贮存1?个月能保持良好的催化活性;通过傅里叶变换红外光谱的变化推断乐果是通过酰胺键连接AuNPs;该方法对环境污染物检测具有潜在的应用价值。

关键词: 纳米金, 过氧化物模拟酶, 有机磷农药检测, 比色分析法

Abstract: This study developed a simple, rapid and visual approach for colorimetric detection of organophosphorus pesticide. Positively-charged gold nanoparticles (AuNPs) with an average diameter of 25 nm were synthesized by seed growth method which possessed intrinsic peroxidase-like activity. AuNPs were demonstrated to have the highest oxidase activity at pH 4.0, 35 ℃ in the presence of 1 mmol/L 3,3’,5,5’-tetramethylbenzidine (TMB) and 10 mmol/L H2O2. It was shown that the nanozyme activity of AuNPs could be inhibited by its surface passivation with rogor, and so an inverse relationship between AuNPs catalytic activity and rogor concentration in the range from 1 to 80 mmol/L was found. The correlation coefficients and sensitivity of the assay were 0.985 and 1.39 mmol/L, respectively. Recoveries from tea samples spiked with different concentrations of rogor were in the range of 91.36%–102.56%. The effective peroxidase-like activity of AuNPs was kept for 1 month at room temperature. Adsorption of rogor onto AuNPs via amide bond was confirmed by Fourier transform infrared (FTIR) spectroscopic analysis. This assay may have potential application in environmental pollutant detection.

Key words: gold nanoparticles, peroxidase mimetics, organophosphorus pesticides, colorimetric analysis

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