50种植物源化合物对阪崎克罗诺肠杆菌的抑菌活性评价

doi:10.7506/spkx1002-6630-201813008

食品科学 ›› 2018, Vol. 39 ›› Issue (13): 47-54.doi: 10.7506/spkx1002-6630-201813008

50种植物源化合物对阪崎克罗诺肠杆菌的抑菌活性评价

石超1，陈怡飞1，贾振宇1，孙怡1，郭都1，杨保伟1，杨华2,*，夏效东1,*

1.西北农林科技大学食品科学与工程学院，陕西杨凌 712100；2.浙江省农业科学院农产品质量标准研究所，浙江杭州 310021

出版日期:2018-07-15 发布日期:2018-07-09
基金资助:
“十二五”国家科技支撑计划项目（2015BAD16B08）；教育部“新世纪优秀人才支持计划”项目（NCET-13-0488）；浙江省植物有害生物防控重点实验室——省部共建国家重点实验室培育基地开放基金项目（2010DS700124-ZM1608）；中央高校基本科研业务费专项资金项目（2452017146）

Evaluation of Antibacterial Activities of Fifty Plant-Derived Compounds against Cronobacter sakazakii

SHI Chao1, CHEN Yifei1, JIA Zhenyu1, SUN Yi1, GUO Du1, YANG Baowei1, YANG Hua2,*, XIA Xiaodong1,*

1. College of Food Science and Engineering, Northwest A & F University, Yangling 712100, China; 2. Institute of Quality and Standard for Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Online:2018-07-15 Published:2018-07-09

摘要/Abstract

摘要： 阪崎克罗诺肠杆菌（Cronobacter sakazkaii）是一种食源性条件致病菌，它能够引起菌血症、坏死性小肠结肠炎和新生儿脑膜炎等多种疾病。本研究选取50 种植物源化合物，检测其在含C. sakazkaii培养基中抑菌圈直径及最小抑菌浓度（minimum inhibitory concentration，MIC），旨在评价植物源化合物对C. sakazakii的抑菌作用并筛查优选高效抑菌剂。结果表明：使C. sakazakii ATCC 29544培养基产生可见抑菌圈的植物源化合物有40 种，其中 7 种（香芹酚、百里醌、百里酚、肉桂醛、柠檬醛、原儿茶醛和原儿茶酸）的抑菌圈平均直径不小于13 mm；在本研究选取的50 种植物源化合物中，百里酚和香芹酚对C. sakazakii有着最强的抑制作用，对9 株C. sakazkaii的MIC均为0.1～0.2 mg/mL；百里醌、肉桂醛、柠檬醛和原儿茶醛对C. sakazkaii有良好的抑菌效果，对C. sakazkaii的MIC为 0.30～1.25 mg/mL；阿魏酸、绿原酸、丁香酸、硫辛酸、原儿茶酸、表儿茶素、咖啡酸、丹皮酚和菊苣酸的MIC为 2.50～5.00 mg/mL。以上结果表明，部分植物源化合物对C. sakazakii有良好的抑菌作用，有潜力作为天然抑菌剂应用于食品加工、流通、贮藏过程中，从而发挥其控制C. sakazakii的作用。

关键词: 阪崎克罗诺肠杆菌, 植物源化合物, 抑菌圈, 最小抑菌浓度

Abstract: Cronobacter sakazakii is an opportunistic pathogen transmitted by foods, which has been implicated in several forms of neonatal infections such as bacteraemia, necrotizing enterocolitis and neonatal meningitis. The aim of this study was to evaluate the antibacterial activity of plant-derived compounds to obtain potent antimicrobials. The antibacterial activity against C. sakazakii was determined by the Oxford cup method. Moreover, the minimum inhibitory concentration (MIC) against four American Type Culture Collection (ATCC) strains and five foodborne isolates was assessed using agar dilution method. Results indicated that 40 phytochemicals had obvious bacteriostatic action against C. sakazakii, and the diameters of the inhibition zones produced by 7 compounds (carvacrol, thymoquinone, thymol, cinnamaldehyde, citral, protocatechuic acid and protocatechualdehyde) were more than 13 mm. Among to 50 compounds tested, thymol and carvacrol had the most potent antimicrobial activity against 9 C. sakazakii strains with MICs ranging from 0.1 to 0.2 mg/mL. Thymoquinone, cinnamaldehyde, citral and protocatechualdehyde also demonstrated good antibacterial effect with MICs of 0.30 to 1.25 mg/mL. The MICs of ferulic acid, chlorogenic acid, syringic acid, lipoid acid, protocatechuic acid, epicatechin, caffeic acid, paeonol and cichoric acid ranged from 2.50 to 5.00 mg/mL. These findings suggest that some plant-derived compounds exhibit good antimicrobial effect against C. sakazakii strains and could be potentially used to control C. sakazakii during food processing, circulation and storage.

Key words: Cronobacter sakazakii, plant-derived compounds, growth inhibition zone, minimum inhibitory concentration

中图分类号:

Q939.92

石超，陈怡飞，贾振宇，孙怡，郭都，杨保伟，杨华，夏效东. 50种植物源化合物对阪崎克罗诺肠杆菌的抑菌活性评价[J]. 食品科学, 2018, 39(13): 47-54.

SHI Chao, CHEN Yifei, JIA Zhenyu, SUN Yi, GUO Du, YANG Baowei, YANG Hua, XIA Xiaodong. Evaluation of Antibacterial Activities of Fifty Plant-Derived Compounds against Cronobacter sakazakii[J]. FOOD SCIENCE, 2018, 39(13): 47-54.

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50种植物源化合物对阪崎克罗诺肠杆菌的抑菌活性评价

Evaluation of Antibacterial Activities of Fifty Plant-Derived Compounds against Cronobacter sakazakii

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