食品科学 ›› 2018, Vol. 39 ›› Issue (21): 160-165.doi: 10.7506/spkx1002-6630-201821024

• 营养卫生 • 上一篇    下一篇

副干酪乳杆菌FM-M9-1对D-半乳糖诱导氧化损伤小鼠的修复机制

王 英,夏秀东,董 月,刘小莉,周剑忠*   

  1. 江苏省农业科学研究院农产品加工研究所,江苏 南京 210014
  • 出版日期:2018-11-15 发布日期:2018-11-21
  • 基金资助:
    国家自然科学基金青年科学基金项目(31501460);南京市科技发展计划项目(201608060)

Mechanism by Which Lactobacillus paracasei FM-M9-1 Repairs D-Galactose-Induced Oxidative Damage in Mice

WANG Ying, XIA Xiudong, DONG Yue, LIU Xiaoli, ZHOU Jianzhong*   

  1. Institute Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
  • Online:2018-11-15 Published:2018-11-21

摘要: 目的:分析副干酪乳杆菌FM-M9-1修复氧化损伤的作用机制。方法:利用D-半乳糖诱导氧化损伤模型小鼠,以高、低剂量((5.01±0.05)×1010、(5.01±0.05)×108 CFU/mL)FM-M9-1活菌体灌胃小鼠,以VC作为阳性药物对照,60 d后测定小鼠的肾和肝组织中超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力及丙二醛、蛋白质羰基含量,同时利用荧光实时定量聚合酶链式反应法分析小鼠肝脏和肾脏组织Sod、Gsh-Px、Nrf2和Trx基因的表达水平。结果:FM-M9-1能不同程度地提高小鼠肝和肾组织中的GSH-Px和SOD活力,降低蛋白质羰基和丙二醛含量,提高与抗氧化功能相关的Sod、Gsh-Px、Nrf2和Trx基因的表达水平。结论:FM-M9-1上调小鼠体内抗氧化相关基因Sod、Gsh-Px、Nrf2和Trx的表达,提高抗氧化酶SOD、GSH-Px活力,抑制体内的蛋白质和脂肪的氧化损伤,最终达到修复小鼠体内氧化损伤的目的。

关键词: 副干酪乳杆菌, D-半乳糖, 小鼠, 氧化损伤, 修复机制

Abstract: Objective: To analyze the mechanism by which Lactobacillus paracasei FM-M9-1 repairs oxidative damage in mice. Methods: A mouse model of oxidative damage was induced by D-galactose. The model mice were gavaged with strain FM-M9-1 at low and high doses of (5.01 ± 0.05) × 1010 and (5.01 ± 0.05) × 108 CFU/mL for 60 successive days, separately. Vitamin C was used as a positive control drug. Afterwards, the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and the contents of malondialdehyde (MDA) and protein carbonyl groups in renal and hepatic tissues were measured. We also examined the expression levels of Sod, Gsh-Px, Nrf2 and Trx by real-time fluorescent quantitative polymerase chain reaction (qPCR). Results: The administration of FM-M9-1 significantly enhanced SOD and GSH-Px activities and reduced MDA and protein carbonyl contents. At the transcriptional level, qPCR analysis suggested that the gene expression of Sod, Gsh-Px, Nrf2 and Trx in mice administered with FM-M9-1 increased. Conclusion: Strain FM-M9-1 can alleviate oxidative stress by increasing the activities of antioxidant enzymes, inhibiting lipid and protein oxidation, and up-regulating the expression of antioxidant function-related genes. Overall, strain FM-M9-1 is a promising probiotic candidate for application in functional foods and antioxidant supplements.

Key words: Lactobacillus paracasei, D-galactose, mice, oxidative damage, repair mechanism

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