食品科学 ›› 2020, Vol. 41 ›› Issue (3): 205-211.doi: 10.7506/spkx1002-6630-20190124-308

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调环酸钙协同硝酸钙控制采后富士苹果苦痘病

彭勇,王君晓,马玉荣,刘佩,王庆国   

  1. (山东农业大学食品科学与工程学院,国家苹果工程技术研究中心,山东 泰安 271018)
  • 出版日期:2020-02-15 发布日期:2020-02-26
  • 基金资助:
    山东省“双一流”奖补资金资助项目(SYT2017XTTD04);山东省自然科学基金面上项目(ZR2017MC051)

Inhibitory Effect of Prohexadione-Calcium Combined with Calcium Nitrate on Bitter Pit of Fuji Apple

PENG Yong, WANG Junxiao, MA Yurong, LIU Pei, WANG Qingguo   

  1. (College of Food Science and Engineering, Shandong Agricultural University, National Research Center for Apple Engineering and Technology, Tai’an 271018, China)
  • Online:2020-02-15 Published:2020-02-26

摘要: 为了控制苹果采后苦痘病的发生,本实验以富士苹果为试材,在水压条件下进行调环酸钙、硝酸钙及二者结合浸钙处理,研究了不同处理间苦痘病的发生率、总钙和不同形态钙含量、抗氧化酶活力等的差异,探讨调环酸钙协同硝酸钙控制苦痘病的可能机理。结果表明:单独调环酸钙处理不能减轻苹果苦痘病的发生,而硝酸钙、调环酸钙/硝酸钙协同处理后,富士苹果贮藏40 d,苦痘病发病率分别比对照低15.62%和50.00%。进一步研究表明协同处理可显著提高苹果外皮层中总钙、水溶性钙含量,在贮藏后期降低外皮层的磷酸钙、草酸钙非生理活性钙含量。此外,调环酸钙协同硝酸钙处理能够显著提高苹果外皮层中2,2’-联氮-双(3-乙基苯并噻唑啉-6-磺酸)阳离子自由基清除率、超氧化物歧化酶活力,抑制组织丙二醛含量的增加,避免果实受到钙胁迫造成的氧化应激损伤。

关键词: 富士苹果, 苦痘病, 调环酸钙, 硝酸钙

Abstract: In order to control the occurrence of bitter pit in postharvest apple, Fuji apples were treated under water pressure with prohexadione-calcium, calcium nitrate and their combination. Changes in bitter pit incidence, total calcium and calcium species contents and antioxidant enzyme activities were investigated, and the mechanism underlying the control of bitter pit by prohexadione-calcium combined with calcium nitrate was discussed. The results showed that prohexadione-calcium alone could not alleviate the occurrence of bitter pit in Fuji apple, while the incidence of bitter pit in apples treated with calcium nitrate alone and in combination with prohexadione-calcium after storage for 40 days was decreased by 15.62% and 50.00%, respectively, when compared with the control. Furthermore, the combined treatment significantly increased the content of total calcium and water-soluble calcium in apple skin, and decreased the content of non-physiological active calcium such as calcium phosphate and calcium oxalate at the later stage of storage. In addition, the combined treatment significantly improved 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) cation radical scavenging activity and superoxide dismutase activity, and inhibited the increase in malondialdehyde (MDA) content in apple skin, thus protecting against oxidative injury caused by calcium stress.

Key words: Fuji apple, bitter pit, prohexadione-calcium, calcium nitrate

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