食品科学 ›› 2018, Vol. 39 ›› Issue (9): 207-214.doi: 10.7506/spkx1002-6630-201809032

• 包装贮运 • 上一篇    下一篇

1-甲基环丙烯结合ξ-聚赖氨酸对贮后货架期富士苹果的品质及挥发性成分的影响

颜廷才1,秦 骅1,张 鹏2,田世平3,李江阔2,*,李博强3   

  1. 1.沈阳农业大学食品学院,辽宁 沈阳 110866;2.国家农产品保鲜工程技术研究中心(天津),天津市农产品采后生理与贮藏保鲜重点实验室,天津 300384;3.中国科学院植物研究所资源植物研发重点实验室,北京 100093
  • 出版日期:2018-05-15 发布日期:2018-05-15
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2016YFD0400903);天津市农业科学院农业部农产品贮藏保鲜重点实验室开放基金项目(KF-08)

Effects of 1-Methylcyclopropene Combined with ξ-Polylysine on Quality and Volatile Components of Fuji Apples during Shelf Life after Cold Storage

YAN Tingcai1, QIN Hua1, ZHANG Peng2, TIAN Shiping3, LI Jiangkuo2,*, LI Boqiang3   

  1. 1. College of Food Science, Shenyang Agricultural University, Shenyang 110866, China; 2. Tianjin Key Laboratory of Postharvest Physiology and Storage of Agricultural Products, National Engineering and Technology Research Center for Preservation of Agricultural Products (Tianjin), Tianjin 300384, China; 3. Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
  • Online:2018-05-15 Published:2018-05-15

摘要: 以富士苹果为实验材料,采后立即用1-甲基环丙烯(1-methylcyclopropene,1-MCP)(1 μL/L)处理后在 精准温控库(-(0.5±0.3)℃)贮藏10 个月后出库,再用不同质量浓度的ξ-聚赖氨酸(500、1 000、1 500 mg/L) 进行喷雾处理,研究1-MCP结合不同质量浓度ξ-聚赖氨酸对贮后常温货架期间苹果品质的影响,采用顶空固相微 萃取-气相色谱-质谱(head space solid-phase microextraction with gas chromatography-mass spectrometry,HS-SPMEGC- MS)联用技术分析不同处理间的挥发性成分变化规律。结果表明:与未经过处理的对照相比,1-MCP结合 ξ-聚赖氨酸处理有效维持了果实的硬度,减缓了苹果的可溶性固形物、可滴定酸质量分数和VC含量的降低,其中 1 000 mg/L的ξ-聚赖氨酸处理效果最好。利用HS-SPME-GC-MS检测出苹果的主要挥发性物质有正己醇、正己醛、 反式-2-己烯醛、乙酸-2-甲基丁酯等。结果表明:对于醛类物质,CK组相对含量先上升后下降,货架末期低于初 期;1 000、1 500 mg/L处理组呈现上升趋势,1-MCP处理组先下降后上升,500 mg/L处理组先上升后下降,且这两 组的醛类物质相对含量在货架末期均高于初期。对于酯类物质,CK、1 000、1 500 mg/L处理组相对含量呈现上升趋 势,500 mg/L处理组先下降后上升,1-MCP处理组先上升后下降。对于醇类物质,1-MCP、1 500 mg/L处理组相对含 量先下降后上升,CK组、500 mg/L处理组上升,1 000 mg/L处理组下降。货架期间,1 000 mg/L处理组的醛类物质相 对含量始终最高,且该处理有效减缓了酯类主要呈味物质乙酸2-甲基丁酯、醇类主要呈味物质2-甲基-1-丁醇相对含量 的下降。1-MCP结合ξ-聚赖氨酸处理能较好地保持苹果的品质和风味,1 000 mg/L的ξ-聚赖氨酸效果最为显著。

关键词: ξ-聚赖氨酸, 1-甲基环丙烯, 富士苹果, 贮后品质, 挥发性成分

Abstract: Fuji apples were treated with 1-Methylcyclopropene (1-MCP) (1 μL/L) immediately after being harvested, and then stored in a precise temperature-controlled refrigerator (?(0.5 ± 0.3)℃) for 10 months. Thereafter, they were transferred to ambient temperature and sprayed with ξ-polylysine at different concentrations (500, 1 000 and 1 500 mg/L). Our purpose was to investigate the effect of 1-MCP combined with ξ-polylysine on the quality of apples during shelf life. The volatile constituents of different treatments were analyzed by headspace solid phase microextraction gas combined with gas chromatographymass spectrometry (HS-SPME-GC-MS). The results showed that 1-MCP combined with ξ-polylysine treatments effectively maintained fruit firmness and slowed down the decrease in the contents of soluble solids, titratable acid and VC in apples when compared with the untreated control and the best effect was observed at an ξ-polylysine concentration of 1 000 mg/L. The main volatile substances identified included hexanol, hexanal, trans-2-hexenal, 2-methylbutyl acetate. The relative content of aldehydes in the CK group increased first, then decreased, and finally was lower at the end than at the beginning of shelf life. The treatment with ξ-polylysine at 1 000 and 1 500 mg/L showed an upward trend; 1-MCP treatment alone resulted in an initial decrease followed by an increase in the relative content of aldehydes, whereas the opposite was observed for the 500 mg/L ξ-polylysine group. The latter two groups showed a higher relative content of aldehydes at the end than at the beginning of shelf life. The relative contents of esters in the CK, 1 000 and 1 500 mg/L treatment groups showed an upward trend. The 500 mg/L treatment group tended to decrease initially and then increased, while the single 1-MCP treatment group showed the opposite trend. The relative content of alcohols in the 1-MCP and 1 500 mg/L ξ-polylysine groups decreased first and then increased, and the CK and 500 mg/L treatment groups and the 1 000 mg/L treatment group showed an increasing and decreasing trend, respectively. During the shelf life, the relative content of aldehydes in the 1 000 mg/L treatment group was always highest, and this treatment could effectively mitigate the decrease in the relative contents of the main flavoractive compounds 2-methyl butyl acetate and 2-methyl-1-butanol. 1-MCP combined with ξ-polylysine treatment could maintain the quality and flavor of apples and ξ-polylysine was most effective when used at 1 000 mg/L.

Key words: ξ-polylysine, 1-methylcyclopropene, Fuji apples, storage quality, volatile components

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