食品科学 ›› 2020, Vol. 41 ›› Issue (9): 294-300.doi: 10.7506/spkx1002-6630-20190411-147

• 专题论述 • 上一篇    下一篇

食品中叶酸测定方法的研究进展

王博伦,顾丰颖,刘子毅,张帆,刘昊,杨婷婷,王锋   

  1. (中国农业科学院农产品加工研究所,农业农村部农产品加工重点实验室,北京 100193)
  • 出版日期:2020-05-15 发布日期:2020-05-15
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2017YFC1600602);中国农业科学院协同创新项目(CAAS-XTCX2016009); 农业部大宗粮食加工重点实验室开放基金项目(DZLS201707)

Advances in Methods for the Determination of Folate in Foods

WANG Bolun, GU Fengying, LIU Ziyi, ZHANG Fan, LIU Hao, YANG Tingting, WANG Feng   

  1. (Key Laboratory of Agro-products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China)
  • Online:2020-05-15 Published:2020-05-15

摘要: 叶酸作为一类人体必需的B族维生素,在人体代谢循环中起到十分重要的作用。人体不能自身合成叶酸,只能从食品中摄取,叶酸缺乏症是全世界范围公认的保健问题,世界卫生组织明确提出了每日叶酸推荐摄入量。天然食品中叶酸含量普遍较低,一些国家进而在谷物制品、婴幼儿奶粉中添加人工合成叶酸;天然强化叶酸的特殊膳食、保健食品也越来越多。因此食品中叶酸含量的准确测定十分关键。本文综述了食品中叶酸提取关键环节与常见的分析检测方法,比较了微生物法、液相色谱法、液相色谱-质谱联用法、荧光法、电化学法等优劣势,期望为建立快速、准确测定食品中叶酸的方法提供参考。

关键词: 叶酸, 叶酸衍生物, 提取, 测定, 食品

Abstract: Folate, an essential vitamin B, plays a very important role in the metabolism and circulation of the human body. Folate cannot be synthesized by the body and therefore has to be supplied through the diet. Folate deficiency is recognized as a health issue worldwide and the World Health Organization has established the recommended daily intake of folate. However, folate content in natural foods is generally low. Fortification of cereal products and infant milk powder products with synthetic folic acid is common practice in many countries; special diets and health food fortified with natural folate have been developed. Therefore, it is crucial to determine the folate content in foods accurately. This paper reviews the key steps of folate extraction from foods and the common analytical methods used for folate detection, and it compares the advantages and disadvantages of microbiological methods, liquid chromatography, liquid chromatography-mass spectroscopy, fluorescence spectroscopy, and electrochemical methods. This review is expected to provide a reference for research aiming to establish a fast and accurate method for the determination of folate in foods.

Key words: folate, folic acid derivative, extraction, determination, foods

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