马尾藻岩藻聚糖分离纯化及其对小鼠黑尾血栓的效果

doi:10.7506/spkx1002-6630-20190519-211

食品科学 ›› 2020, Vol. 41 ›› Issue (9): 91-97.doi: 10.7506/spkx1002-6630-20190519-211

马尾藻岩藻聚糖分离纯化及其对小鼠黑尾血栓的效果

刘海韵，王维民，谌素华，吕佳桐，陈华英，廖森泰

（1.广东海洋大学食品科技学院，广东省水产品加工与安全重点实验室，广东湛江 524088；2.广东省农业科学院蚕业与农产品加工研究所，广东广州 510610）

出版日期:2020-05-15 发布日期:2020-05-15
基金资助:
广东省自然科学基金项目（2018A0303130020）；2015年广东省联合培养研究生示范基地项目（粤教研函[2016]1号）

Isolation and Purification of Fucoidans from Sargassum and Their Effect on Tail Thrombosis in Mice

LIU Haiyun, WANG Weimin, CHEN Suhua, Lü Jiatong, CHEN Huaying, LIAO Sentai

(1. Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China; 2. Sericulture & Farm Produce Processing Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China)

Online:2020-05-15 Published:2020-05-15

摘要/Abstract

摘要： 研究3 种马尾藻岩藻聚糖组分（F、FD1、FS1）对小鼠黑尾血栓的效果。运用超声波辅助热水浸提法从马尾藻中提取并经过醇沉、Sevag试剂脱蛋白等初级纯化方法制得粗多糖（F）。将F过DEAE C-52纤维素层析柱得次级纯化组分（FD1），将F过Sepharose CL-6B琼脂糖凝胶层析柱得次级纯化组分（FS1）。对3 种岩藻聚糖组分进行化学组成分析并运用高效液相色谱测定其单糖组成。建立小鼠黑尾模型以评价3 种组分的血栓抑制效果，并采用毛细管法测定凝血时间。结果表明：经过层析柱纯化后组分的多糖质量分数均有不同程度的提升，FD1的硫酸基质量分数最高，FS1的岩藻糖质量分数最高。3 种组分单糖组成情况差异明显，但均不含阿拉伯糖。FS1组分最为纯净。对于各组分的摄入并不会影响小鼠的体质量，说明岩藻聚糖是安全的。不同组分的各剂量组小鼠黑尾长度均短于模型对照组，且纯化后的FD1和FS1抑制小鼠血栓的效果均比粗多糖F更好，60 mg/kg mb的FD1组和15 mg/kg mb的FS1组抑制血栓的效果极显著（P＜0.01）。同时各组分均可延长小鼠的凝血时间，效果依次为FS1＞FD1＞F，FD1中、高剂量组和FS1 3 个剂量组的凝血时间均较阳性药物组更长。

关键词: 马尾藻, 岩藻聚糖, 纯化, 黑尾, 血栓, 凝血

Abstract: The effects of crude (F) and purified (FD1 and FS1) fucoidans from Sargassum on tail thrombosis in mice were studied. F was prepared by ultrasonic-assisted hot water extraction, alcohol precipitation and deproteinization using Sevag reagent. FD1 and FS1 were obtained by passing F through a DEAE C-52 cellulose column and a Sepharose CL-6B agarose gel column, respectively. The chemical composition of the three fucoidans was analyzed and their monosaccharide composition was determined by high performance liquid chromatography (HPLC). A mouse black tail model was established and used to evaluate the inhibition effect of the three fucoidans on thrombus formation, and the clotting time was determined by capillary method. The results showed that the polysaccharide content of the crude fucoidans was increased to different extents after being purified by the chromatographic columns. The sulphate content of FD1 was the highest and the fucose content of FS1 was the highest among the three fucoidans. Their monosaccharide composition was significantly different from other, but none contained arabinose. FS1 showed the highest fucoidan content. Ingestion of each fucoidan did not affect the body mass of mice, indicating their safety. The black tail length of mice treated with each fucoidan at all doses investigated was shorter than that of the model control group, and FD1 and FS1 inhibited thrombus formation in mice more effectively than F, with a significant difference being observed for FD1 at 60 mg/kg mb and FS1 at 15 mg/kg mb (P < 0.01). All three fucoidans could prolong the clotting time of mice in the order of decreasing effectiveness: FS1> FD1> F, and the clotting time of the high-dose FD1 group and the high-, medium- and low-dose FS1 groups were longer than that of the positive drug group.

Key words: Sargassum, fucoidan, purification, black tail, thrombus, coagulation

中图分类号:

TS254.1

刘海韵，王维民，谌素华，吕佳桐，陈华英，廖森泰. 马尾藻岩藻聚糖分离纯化及其对小鼠黑尾血栓的效果[J]. 食品科学, 2020, 41(9): 91-97.

LIU Haiyun, WANG Weimin, CHEN Suhua, Lü Jiatong, CHEN Huaying, LIAO Sentai. Isolation and Purification of Fucoidans from Sargassum and Their Effect on Tail Thrombosis in Mice[J]. FOOD SCIENCE, 2020, 41(9): 91-97.

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马尾藻岩藻聚糖分离纯化及其对小鼠黑尾血栓的效果

Isolation and Purification of Fucoidans from Sargassum and Their Effect on Tail Thrombosis in Mice

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