食品科学 ›› 2020, Vol. 41 ›› Issue (22): 324-329.doi: 10.7506/spkx1002-6630-20190714-182

• 安全检测 • 上一篇    下一篇

基于特异性适配体封盖介孔纳米材料的T-2毒素快速检测技术

徐群博,谭红霞,马良   

  1. (西南大学食品科学学院,重庆 400715)
  • 出版日期:2020-11-25 发布日期:2020-11-26
  • 基金资助:
    国家级大学生创新创业训练计划项目(201910635029); 重庆市技术创新与应用示范(社会民生类)项目(cstc2018jscx-msybX0204)

A Rapid Technique for the Detection of T-2 Toxin Based on Specific Aptamer-Capped Mesoporous Nanomaterials

XU Qunbo, TAN Hongxia, MA Liang   

  1. (College of Food Science, Southwest University, Chongqing 400715, China)
  • Online:2020-11-25 Published:2020-11-26

摘要: 利用介孔纳米材料富集信号探针,采用T-2毒素适配体作为识别探针和信号探针的控制开关,检测体系中的靶标T-2毒素可以与识别探针T-2毒素适配体进行特异性结合并释放出信号探针,根据信号强度与靶标毒素含量的相关关系建立一种非标记型T-2毒素快速定量检测技术。结果表明,应用本法对玉米、大麦、小麦等样品检测加标回收率为85.8%~111.4%,相对标准偏差在4.6%以内,具有良好的检测准确性和重复性;同时该方法灵敏度高,方法检出限为1.25 ng/mL,且在不同污染范围内(0~50、50~250 ng/mL)线性相关性较强,与传统的实验方法相比,该方法具有快速、简便、廉价等优点,可在较宽的线性范围内进行T-2毒素的高灵敏度定量检测。

关键词: T-2毒素;适配体;介孔二氧化硅纳米颗粒;非标记;荧光

Abstract: In this paper, using mesoporous silica nanoparticles (MSN) to enrich the signal probe and using the T-2 toxin aptamer as a recognition probe and a switch to control the signal probe, a rapid label-free technique for the quantitative detection of T-2 toxin was proposed based on the relationship between the signal intensity and the content of the target toxin. In the detection system, the target T-2 toxin could specifically bind to its aptamer, releasing the signal probe. The results showed that when this method was applied to corn, barley and wheat samples, the spiked recoveries of T-2 toxin were in the range between 85.8% and 111.4% with relative standard deviation (RSD) less than 4.6%, indicating that this method has good accuracy and repeatability. The method was sensitive with a limit of detection of 1.25 ng/mL and showed a good linearity in the concentration range of 0–50 and 50–250 ng/mL. Compared with the traditional method, this novel method was more rapid, simpler and cheaper and allowed sensitive and quantitative detection of T-2 toxin with a wider linear range.

Key words: T-2 toxin; aptamer; mesoporous silica nanoparticles; label-free; fluorescence spectroscopy

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