食品科学 ›› 2020, Vol. 41 ›› Issue (16): 173-178.doi: 10.7506/spkx1002-6630-20190827-290

• 成分分析 • 上一篇    

新鲜草果中关键香气成分的分析

胡智慧,白佳伟,杨文熙,刘玉平,孙宝国   

  1. (1.北京食品营养与人类健康高精尖创新中心,北京工商大学,北京 100048;2.北京市食品风味化学重点实验室,北京 100048;3.北京工商大学食品与健康学院,食品质量与安全北京实验室,北京 100048)
  • 发布日期:2020-08-19
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2017YFD0400501)

Identification of the Key Odorants in Fresh Amomum tsaoko Fruit

HU Zhihui, BAI Jiawei, YANG Wenxi, LIU Yuping, SUN Baoguo   

  1. (1. Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, China; 2. Beijing Key Laboratory of Flavour Chemistry, Beijing 100048, China; 3. Beijing Laboratory for Food Quality and Safety, School of Food and Health, Beijing Technology and Business University, Beijing 100048, China)
  • Published:2020-08-19

摘要: 采用溶剂直接萃取结合溶剂辅助风味成分蒸发的方法,提取产自云南怒江的新鲜草果壳和种仁中挥发性成分,使用气相色谱-质谱-嗅觉检测器联用仪对所得提取物进行分析。通过与标准品的香气特征、质谱、保留指数进行比对,从中鉴定出30 种香气活性成分,包括烯烃13 种、醇10 种、醛6 种、醚1 种。以2-辛醇和2-异丙基苯酚为内标,对27 个香气活性成分进行定量;使用定量结果和阈值,计算其中22 种成分的香气活性值。根据香气活性值,确定出新鲜草果壳和种仁中关键香气成分分别为17 种和16 种;其中1,8-桉叶素、(+)-α-蒎烯、反-2-辛烯醛、反-2-癸烯醛、β-罗勒烯、芳樟醇、α-松油醇、榄香醇、反-橙花叔醇、β-蒎烯、桧烯和4-萜烯醇是新鲜草果壳和种仁共有的关键香气物质,它们使二者香气具有相似之处;而香叶醛、α-水芹烯、橙花醛、萜品油烯、反-2-十二烯醛、β-月桂烯、正己醛、d-柠檬烯和β-石竹烯是导致二者香气有所不同的主要成分。

关键词: 新鲜草果;溶剂辅助风味成分蒸发;香气活性成分;关键香气成分

Abstract: The volatile components in the pericarps and seed kernels of fresh Amomum tsaoko (ATK) from Nujiang, Yunnan were extracted by direct solvent extraction combined with solvent-assisted flavor evaporation (SAFE), and were analyzed by gas chromatography-mass spectrometry-olfactometry (GC-MS-O). Thirty odor-active components (OACs), including 13 alkenes, 10 alcohols, 6 aldehydes and 1 ether, were identified by comparing the odor characteristics, mass spectrometry (MS) data, and retention indices with those of authentic compounds. Twenty-seven OACs were quantitated by using 2-octanol and 2-isopropylphenol as the internal standards. According to the quantitative results and the thresholds of OACs, the odor activity values (OAVs) of 22 OACs were calculated. Based on the OAVs obtained, a total of 17 key odorants (KOs) in the pericarps and 16 KOs in the seed kernels were determined. Some KOs, including 1,8-cineole, (+)-α-pinene, (E)-2-octenal, (E)-2-decenal, β-ocimene, linalool, α-terpineol, elemol, (E)-nerolidol, β-pinene, sabinene and 4-terpineol, were common to the pericarps and seed kernels, contributing to their similarities in odor notes. However, some odorants, including geranial, α-phellandrene, neral, terpinolene, (E)-2-dodecenal, β-myrcene, hexanal, d-limonene and β-caryophyllene, only occurred in either the pericarps or kernels, which made a distinction between their odors.

Key words: fresh fruit of Amomum tsaoko Crevost et Lemaire; solvent-assisted flavor evaporation; odor-active compounds; key odorants

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