食品科学 ›› 2021, Vol. 42 ›› Issue (20): 98-106.doi: 10.7506/spkx1002-6630-20201022-224

• 成分分析 • 上一篇    下一篇

基于感官检验和气相色谱-质谱联用对白芽奇兰茶叶香气分级

蒋青香,李慧雪,李利君,黄高凌,倪辉   

  1. (1.集美大学海洋食品与生物工程学院,福建 厦门 361021;2.福建省食品微生物与酶工程重点实验室,福建 厦门 361021;3.厦门市食品生物工程技术研究中心,福建 厦门 361021)
  • 出版日期:2021-10-25 发布日期:2021-11-12
  • 基金资助:
    国家自然科学基金面上项目(31871765);福建省高校产学合作项目(2020N5010)

Aroma Classification of Baiyaqilan Tea by Sensory Test and Gas Chromatography-Mass Spectrometry

JIANG Qingxiang, LI Huixue, LI Lijun, HUANG Gaoling, NI Hui   

  1. (1. College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China; 2. Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen 361021, China; 3. Research Center of Food Biotechnology of Xiamen City, Xiamen 361021, China)
  • Online:2021-10-25 Published:2021-11-12

摘要: 为了解不同等级白芽奇兰茶叶的香气品质差异及其物质基础,采用定量描述分析(quantitative descriptive analysis,QDA)、顶空固相微萃取(headspace solid phase microextraction,HS-SPME)结合气相色谱-质谱联用仪(gas chromatography-mass spectrometry,GC-MS)对4 种白芽奇兰茶叶的香气品质进行分析,并运用多元逐步回归分析、Pearson线性相关性进一步分析不同香气属性强度和挥发性成分含量与可接受程度的关系。QDA表明白芽奇兰茶叶水溶液的整体香气轮廓为花香、青草香、甜香、烘烤香、木香和焦糖香,但不同白芽奇兰茶叶水溶液的香气属性强度和可接受程度不同。GC-MS检测结果表明:不同白芽奇兰茶叶中,其挥发性成分的组成和含量有一定差异;在4 种茶叶中共检测出55 种挥发性成分,其中醇类含量最高,占挥发性成分总含量的46.3%~69.7%;脱氢芳樟醇、吲哚、反式-橙花叔醇、香叶醇、1-乙基-2-甲酰基吡咯及芳樟醇是白芽奇兰茶叶的主要挥发性成分。多元逐步回归分析表明花香、青草香和甜香与可接受程度呈正相关,且发现花香、青草香和甜香与6-甲基-5-乙基-3-庚烯-2-酮、香叶醇、顺式-3-己烯基己酸酯、顺式-茉莉酮、反式-橙花叔醇的含量呈正相关,与二甲基硫醚的含量呈负相关。以上结果阐明了白芽奇兰茶叶香气品质分级的成分指标,为实现白芽奇兰茶叶香气品质客观评价提供了参考。

关键词: 白芽奇兰茶叶;定量描述分析;气相色谱-质谱法;挥发性成分;多元逐步回归分析;Pearson线性相关性;等级划分

Abstract: In order to understand the difference in aroma quality among different grades of Baiyaqilan tea and the material basis for it, the aroma quality of four different Baiyaqilan teas was analyzed by quantitative descriptive analysis (QDA) and headspace solid phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). Multiple stepwise regression analysis and Pearson’s linear correlation were used to further analyze the relationship of different aroma attribute intensities and volatile compound contents with sensory acceptability. The results of QDA showed that the overall aroma profile of Baiyaqilan tea infusion consisted of floral, grassy, sweet, roasted, woody and caramel notes, and the aroma intensity and acceptability of different Baiyaqilan tea infusions were different. The results of GC-MS showed that there were some differences in the types and contents of volatile compounds in different Baiyaqilan teas, and 55 volatile compounds were detected in the four teas investigated, alcohols being the most abundant, accounting for 46.3%–69.7% of the total content of volatile compounds. Hotrienol, indole, (E)-nerolidol, geraniol, 1-ethyl-1H-pyrrole-2-carbaldehyde and linalool were the main volatile compounds in Baiyaqilan tea. Multiple stepwise regression analysis showed that the floral, grassy and sweet notes were positively correlated with the sensory acceptability, and that the floral, grassy and sweet notes were positively correlated with the contents of 6-methyl-5-ethyl-3-heptene-2-one, geraniol, (Z)-3-hexenyl hexanoate, (Z)-jasmone, (E)-nerolidol, and negatively correlated with the content of dimethyl sulfide. The above results may provide a reference for the objective evaluation of Baiyaqilan tea aroma quality.

Key words: Baiyaqilan tea; quantitative descriptive analysis; gas chromatography-mass spectrometry; volatile compounds; multiple stepwise regression analysis; Pearson’s linear correlation; classification

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