食品科学 ›› 2022, Vol. 43 ›› Issue (5): 286-294.doi: 10.7506/spkx1002-6630-20201206-065

• 专题论述 • 上一篇    下一篇

蛋白质组学在肉类真实性鉴别中的应用研究进展

张颖颖,康超娣,张明悦,王娟强,赵文涛,李莹莹   

  1. (北京食品科学研究院,中国肉类食品综合研究中心,北京 100068)
  • 发布日期:2022-03-28
  • 基金资助:
    国家自然科学基金青年科学基金项目(31801639)

Progress in the Application of Proteomics in Meat Product Authentication

ZHANG Yingying, KANG Chaodi, ZHANG Mingyue, WANG Juanqiang, ZHAO Wentao, LI Yingying   

  1. (Beijing Academy of Food Sciences, China Meat Research Center, Beijing 100068, China)
  • Published:2022-03-28

摘要: 基于质谱的蛋白质组学技术已逐渐应用于肉类食品的真实性鉴别中,通过对特异性生物标志物的蛋白或肽段进行鉴定、检测和定量,为确证物种的真实属性、含量及来源提供有效的技术手段。本文简要介绍蛋白质组学工作流程,对物种特异性多肽的提取、分析鉴定、热稳定性评价及筛选原则进行详细概述,列举了该技术在肉类食品的动物源性及非肉蛋白成分检测鉴别中的应用,并对其发展方向及前景进行了展望,以期为复杂的食品体系研究提供新思路,也为食品溯源提供更丰富的技术手段。

关键词: 蛋白质组学;质谱技术;真伪鉴别;食品溯源

Abstract: Mass spectrometry-based proteomics has been gradually applied in the authentication of meat products, which can identify, detect and quantify specific biomarker proteins or peptides, providing an effective technical means for confirming the true attributes, contents and sources of species. This article briefly introduces the proteomics workflow, and gives a detailed overview of the extraction, identification, thermal stability and screening principle of species-specific peptides. Meanwhile, this article describes the application of proteomics in the detection and identification of animal-derived and non-meat protein components in meat products, and discusses prospects for future directions.

Key words: proteomics; mass spectrometry; authentication; food traceability

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