食品科学 ›› 2022, Vol. 43 ›› Issue (4): 127-134.doi: 10.7506/spkx1002-6630-20210410-134

• 成分分析 • 上一篇    下一篇

基于电子舌检测快速判别不同物种畜禽骨蛋白肽

刘泓,郭玉杰,陈永凯,李侠,张鸿儒,韩东,张春晖,姜珊   

  1. (1.中国农业科学院农产品加工研究所,农业农村部农产品加工综合性重点实验室,北京 100193;2.内蒙古阿荣旗牧源康肽实业有限公司,内蒙古 呼伦贝尔 162750)
  • 出版日期:2022-02-25 发布日期:2022-03-08
  • 基金资助:
    国家自然科学基金面上项目(32072156);国家农业科技创新工程项目(CAAS-ASTIP-2021-IFST); 内蒙古自治区重大成果项目(2019BG640243)

Rapid Discrimination of Bone Peptides from Different Livestock and Poultry Species Using Electronic Tongue

LIU Hong, GUO Yujie, CHEN Yongkai, LI Xia, ZHANG Hongru, HAN Dong, ZHANG Chunhui, JIANG Shan   

  1. (1. Comprehensive Key Laboratory of Agro-products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China;2. Inner Mongolia Arong Banner Muyuankangtai Industrial Co. Ltd., Hulun Buir 162750, China)
  • Online:2022-02-25 Published:2022-03-08

摘要: 目的:为深入了解畜禽骨蛋白肽的滋味特征及其差异,探究电子舌快速判别鉴定不同物种畜禽骨蛋白肽的可行性。方法:取牦牛骨蛋白肽(yak bone peptides,YBPs)、黄牛骨蛋白肽(bovine bone peptides,BBPs)、猪骨蛋白肽(pig bone peptides,PBPs)、鸡骨蛋白肽(chicken bone peptides,CBPs)各4 份,共16 份畜禽骨蛋白肽样本,首先分析畜禽骨蛋白肽的基本营养组分及氨基酸组成,明确滋味特征差异的物质基础,其次利用电子舌检测结合多元统计分析对种间畜禽骨蛋白肽的滋味特征数据进行定量判别分析。结果:4 种畜禽骨蛋白肽的基本组分及氨基酸组成存在差异。主成分分析(principal component analysis,PCA)和判别因子分析(discriminant factor analysis,DFA)可有效区分畜禽骨蛋白肽的种间差异,且DFA的判别效果明显优于PCA;偏最小二乘判别分析、正交偏最小二乘判别分析及Fisher判别模型对畜禽骨蛋白肽种间滋味特征的判别能力良好,Fisher模型判别正确率为68.8%。结论:鲜味、甜味和苦味为4 种畜禽骨蛋白肽主要的差异滋味特征,电子舌滋味检测结合多元统计分析能够为畜禽骨蛋白肽判别提供一种快速、可靠的技术手段。

关键词: 电子舌;畜禽骨蛋白肽;多元统计分析;判别

Abstract: Objective: To gain a deep understanding of the taste characteristics of bone peptides from different species of livestock and poultry and the differences between them, and to explore the feasibility of using electronic tongue in their rapid qualitative identification. Methods: Totally 16 samples of yak bone peptides (YBPs), bovine bone peptides (BBPs), pig bone peptides (PBPs) and chicken bone peptides (CBPs), four samples from each species, were collected for analysis of nutritional components and amino acid composition to clarify the material basis of the taste difference between them, and then the data of their taste characteristics were quantitatively discriminated by electronic tongue combined with multivariate statistical analysis. Results: The nutritional composition and amino acid composition of bone peptides from different animal species were different. Principal component analysis (PCA) and discriminant factor analysis (DFA) distinguished bone peptides from different animal species, and DFA was significantly more effective than PCA. Supervised partial least squares discriminant analysis (PLS-DA), orthogonal partial least squares discriminant analysis (OPLS-DA) and Fisher discriminant analysis model all had good discriminant capability for the taste characteristics of bone peptides, and the discriminant accuracy of the Fisher model was 68.8%. Conclusion: The taste characteristics of the four bone peptides differ umami, sweetness and bitterness. Electronic tongue taste detection combined with multivariate statistical analysis can provide a rapid and reliable means for the discrimination of bone peptides from different animal species.

Key words: electronic tongue; bone peptides from livestock and poultry; multivariate statistical analysis; discriminant analysis

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