食品科学 ›› 2023, Vol. 44 ›› Issue (7): 276-285.doi: 10.7506/spkx1002-6630-20220501-001

• 专题论述 • 上一篇    

食品接触表面生物被膜形成机制及防控方法研究进展

马悦,吴梦洁,李卓思,刘阳泰,董庆利   

  1. (上海理工大学健康科学与工程学院,上海 200093)
  • 发布日期:2023-04-25
  • 基金资助:
    国家自然科学基金青年科学基金项目(32102095); 上海市农委2021年度上海市科技兴农技术创新项目(X2021-02-08-00-12-F00782)

Formation Mechanism and Control Strategies of Biofilm on Food Contact Surfaces

MA Yue, WU Mengjie, LI Zhuosi, LIU Yangtai, DONG Qingli   

  1. (School of Health Science and Technology, University of Shanghai for Science and Technology, Shanghai 200093, China)
  • Published:2023-04-25

摘要: 食源性致病菌的生物被膜是固着在食品接触表面上形成的具有一定空间组织的多细胞群体结构。因生物被膜具有极强的黏附性和抗逆性,常规消杀手段难以对其进行有效防控,造成食品安全隐患并严重威胁消费者身体健康。本文归纳了近年来国内外食源性致病菌生物被膜在形成机制及防控方法方面的相关研究。以生物被膜黏附性提高、菌体状态改变和抗逆性增强的3 个主要特点为核心,总结讨论了细菌的长链附属结构、群体感应系统及胞外聚合物在生物被膜的形成过程中的作用,并将生物被膜的防控策略分为物理、化学和生物法3 类,分别分析了各类方法的作用原理及优缺点,旨在为食品领域生物被膜的高效防控方法的开发提供理论指导,以期更好地实现食品微生物的安全有效防控。

关键词: 食源性致病菌;生物被膜;形成机制;防控方法

Abstract: Foodborne pathogenic bacterial biofilm is a spatially structured multi-cellular community that is adhered to food contact surfaces. Conventional disinfection methods cannot effectively prevent and control bacterial biofilm due to its strong adhesiveness and stress resistance, causing food safety hazards and a serious effect on consumer health. In this article, recent studies on the formation mechanism and control strategies of foodborne pathogenic bacterial biofilm are reviewed. The roles of the functions of bacterial long-chain appendages, quorum sensing system and extracellular polymeric substances (EPSs) in the formation of bacterial biofilm are summarized and discussed based on improved biofilm adhesiveness, changed bacterial state, and enhanced stress resistance. This review divides the existing strategies for biofilm prevention and control into three categories: physical, chemical, and biological, and analyzes the principle, advantages and disadvantages of each category in order to provide a theoretical reference for the development of more efficient strategies for biofilm prevention and control in the food field to achieve better prevention and control of food microbial safety.

Key words: foodborne pathogen; biofilm; formation mechanism; prevention and control strategies

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