食品科学 ›› 2023, Vol. 44 ›› Issue (17): 94-100.doi: 10.7506/spkx1002-6630-20220810-124

• 营养卫生 • 上一篇    下一篇

刺梨多酚对NG-硝基-L-精氨酸甲酯诱导小鼠高血压的影响及机制

徐青,谭书明,俞露,袁梦,谭芸芸,张丽群   

  1. (贵州大学酿酒与食品工程学院,贵州省刺梨研究院,贵州 贵阳 550025)
  • 出版日期:2023-09-15 发布日期:2023-09-29
  • 基金资助:
    贵州省刺梨产业研究院平台建设项目(黔财农[2019]261);贵州省工业和信息化厅项目(黔财农[2020]307)

Effect and Mechanism of Rosa roxburghii Fruit Polyphenols on Hypertension Induced by NG-Nitro-L-Arginine Methyl Ester in Mice

XU Qing, TAN Shuming, YU Lu, YUAN Meng, TAN Yunyun, ZHANG Liqun   

  1. (School of Liquor and Food Engineering, Guizhou University, Guizhou Rosa Roxburghii Research Institute, Guiyang 550025, China)
  • Online:2023-09-15 Published:2023-09-29

摘要: 目的:探讨刺梨多酚(Rosa roxburghii fruit polyphenols,RRP)对NG-硝基-L-精氨酸甲酯(NG-nitro-L-arginine methyl ester,L-NAME)诱导小鼠高血压的影响及其可能的作用机制。方法:实验室自制得到纯度为68%的RRP;采用L-NAME诱导小鼠高血压模型,将小鼠分为空白组、模型组、RRP低剂量组、RRP高剂量组,解剖后测定脏器指数,保存血清用于测定一氧化氮(nitric oxide,NO)、内皮素-1(endothelin-1,ET-1)、肾素、血管紧张素II(angiotensin II,Ang II)、醛固酮(aldosterone,ALD)、白细胞介素-6(interleukin 6,IL-6)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)水平,肾脏和肝脏组织用于测定氧化应激标志物丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)和谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)水平,心脏和肾脏样本用苏木精-伊红染色并观察病理学变化。结果:与空白组相比,模型组血压明显升高;血清NO浓度显著降低(P<0.05),血清IL-6、TNF-α水平显著升高(P<0.05),血清ET-1、肾素、Ang II、ALD水平显著升高(P<0.05);肾脏和肝脏MDA含量显著升高(P<0.05),SOD、GSH-Px活力显著降低(P<0.05)。与模型组相比,阳性组及RRP低、高剂量组血压在实验末期显著降低(P<0.05);血清NO浓度显著升高,IL-6、TNF-α水平显著降低(P<0.05),ET-1、肾素、Ang II、ALD水平显著降低(P<0.05);肾脏和肝脏MDA含量显著降低(P<0.05),GSH-Px活力显著升高(P<0.05)。结论:RRP具有降血压作用,这可能与RRP能够提高NO水平、改善内皮细胞功能障碍、抑制肾素-血管紧张素-醛固酮系统过度激活、减轻炎症和氧化应激有关。

关键词: 刺梨多酚;高血压;内皮功能;肾素-血管紧张素-醛固酮系统;炎症;氧化应激

Abstract: Objective: To investigate the effect and possible mechanism of Rosa roxburghii fruit polyphenols (RRP) on hypertension induced by NG-nitro-L-arginine methyl ester (L-NAME) in mice. Methods: RRP with a purity of 68% was prepared in the laboratory. L-NAME was used to induce a mouse model of hypertension, and the mice were divided into four groups: model, positive control (captopril), low-dose RRP, and high-dose RRP. All mice were killed and dissected for measurement of organ indexes. Mice not treated with L-NAME served as a control group. The serum was collected to determine the levels of nitric oxide (NO), endothelin-1 (ET-1), renin, angiotensin II (Ang II), aldosterone (ALD), interleukin 6 (IL-6), and tumor necrosis factor-α (TNF-α). The levels of oxidative stress markers including malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were assayed in kidney and liver tissues, and heart and kidney tissues were stained with hematoxylin and eosin (HE) for pathological observation. Results: Compared with the blank group, the model group showed a significant increase in blood pressure; serum NO concentration significantly decreased (P < 0.05), serum IL-6 and TNF-α levels significantly increased (P < 0.05), and serum ET-1, renin, Ang II, and ALD levels significantly increased (P < 0.05). MDA levels in kidney and liver tissues significantly increased (P < 0.05), and SOD and GSH-Px activities significantly decreased (P < 0.05). Compared with the model group, blood pressure significantly increased in the positive control group and the low- and high-dose RRP groups (P < 0.05). Serum NO levels significantly increased (P < 0.05), and serum IL-6, TNF-α, ET-1, renin, Ang II, and ALD levels significantly decreased (P < 0.05). MDA levels in kidney and liver tissues significantly declined (P < 0.05), and GSH-Px activity significantly increased (P < 0.05). Conclusion: RRP has an obvious hypotensive effect, possibly by increasing NO bioavailability, improving endothelial cell dysfunction, inhibiting the over-activation of the renin angiotensin aldosterone system, and reducing inflammation and oxidative stress.

Key words: Rosa roxburghii fruit polyphenols; hypertension; endothelial function; renin angiotensin aldosterone system; inflammation; oxidative stress

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