食品科学 ›› 2023, Vol. 44 ›› Issue (17): 101-109.doi: 10.7506/spkx1002-6630-20220906-055

• 营养卫生 • 上一篇    下一篇

山楂多酚缓解苯并芘诱导的呼吸道上皮细胞炎性损伤活性

燕霞凤,侯召勤,张翠芬,马燕飞,王建军   

  1. (1.东营市人民医院科教科,山东 东营 257000;2.深圳大学 呼吸疾病国家重点实验室深圳大学变态反应分室,广东 深圳 518060)
  • 出版日期:2023-09-15 发布日期:2023-09-29
  • 基金资助:
    山东省医药卫生科技发展计划项目(202012070681)

Hawthorn Polyphenols Relieve Benzo(a)pyrene-Induced Inflammatory Injury in Respiratory Epithelial Cells

YAN Xiafeng, HOU Zhaoqin, ZHANG Cuifen, MA Yanfei, WANG Jianjun   

  1. (1. Science and Education Department of Dongying People’s Hospital, Dongying 257000, China; 2. State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen 518060, China)
  • Online:2023-09-15 Published:2023-09-29

摘要: 目的:基于苯并芘(benzo(a)pyrene,BaP)暴露的人支气管上皮(16HBE)细胞模型评价山楂活性多酚(hawthorn bioactive polyphenols,HBPs)细胞保护活性及潜在机制。方法:基于BaP暴露的16HBE细胞模型采用活性追踪方法分离HBPs;噻唑蓝(methyl thiazolyl tetrazolium,MTT)法检测HBPs细胞毒性,CCK-8法检测HBPs对BaP诱导的16HBE细胞损伤的细胞保护活性;酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)法检测化合物1(HBP-1)对BaP诱导的16HBE细胞炎症因子(肿瘤坏死因子α(tumor necrosis factor alpha,TNF-α)、白细胞介素-1β(interleukin-1β,IL-1β)、IL-18、IL-10、IL-6)及细胞内活性氧(reactive oxygen species,ROS)的影响;流式细胞术检测HBP-1对BaP诱导的16HBE细胞凋亡的影响;Western blotting检测HBP-1对BaP诱导的16HBE细胞芳香烃受体(aryl hydrocarbon receptor,AhR)及核因子-κB(nuclear factor kappa-B,NF-κB)信号通路蛋白表达的影响。结果:从山楂中共分离出10 个活性酚类化合物,其中,HBP-1对BaP暴露导致的16HBE细胞炎性损伤的细胞保护活性最佳。HBP-1能够显著抑制BaP诱导的16HBE细胞炎症因子(IL-1β、IL-18及TNF-α)过量分泌及细胞内ROS水平升高;并抑制BaP诱导的16HBE细胞凋亡;Western blotting结果显示,HBP-1能够抑制BaP诱导的16HBE细胞AhR及NF-κB信号通路的激活。结论:HBP-1可通过抑制AhR及NF-κB信号通路的激活,抑制BaP诱导的16HBE细胞氧化应激及炎症因子过量分泌,从而发挥其细胞保护活性。

关键词: 山楂;苯并芘;多酚;芳香烃受体;核因子-κB

Abstract: Objective: The objective of this study is to evaluate the cytoprotective activity and potential mechanism of hawthorn bioactive polyphenols (HBPs) using human bronchial epithelial (16HBE) cells exposed to benzo(a)pyrene (BaP). Methods: HBPs were isolated by activity-guided separation, and their cytotoxicity was assessed by the methyl thiazolyl tetrazolium (MTT) method. The cytoprotective activity of HBPs against BaP-induced injury in 16HBE cells was determined by the cell counting kit-8 (CCK-8) method. Enzyme-linked immunosorbent assay (ELISA) was performed to detect the effect of HBP-1 on BaP-induced inflammatory factors such as tumor necrosis factor alpha (TNF-α), interleukin-1β (IL-1β), IL-18, IL-10, IL-6 and reactive oxygen species (ROS) in 16HBE cells. Flow cytometry was utilized to explore the effect of HBP-1 on BaP-induced apoptosis in 16HBE cells. Western blotting was conducted to examine the effect of HBP-1 on protein expression related to the aryl hydrocarbon receptor (AhR) and nuclear factor kappa-B (NF-κB) signaling pathways in BaP-induced 16HBE cells. Results: A total of 10 bioactive phenolic compounds were isolated from hawthorn. Among them, HBP-1 exhibited the most significantly cytoprotective activity against BaP-induced inflammatory injury in 16HBE cells. HBP-1 significantly inhibited the oversecretion of IL-1β, IL-18 and TNF-α and the increase in ROS level and BaP-induced apoptosis in 16HBE cells. Western blotting results indicated that HBP-1 could inhibit the activation of the AhR and NF-κB signaling pathways in 16HBE cells induced by BaP. Conclusion: HBP-1 is able to inhibit BaP-induced oxidative stress and excessive production of inflammatory cytokines in 16HBE cells by suppressing the activation of the AhR and NF-κB signaling pathways, highlighting the cytoprotective effect of HBP-1 against BaP-induced damage.

Key words: hawthorn; benzoapyrene; polyphenol; aryl hydrocarbon receptor; nuclear factor kappa-B

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