关键词: 岩藻糖基寡糖；婴儿肠道菌群；双歧杆菌；短链脂肪酸；三代测序

Abstract: The effects of the degradation products of fucosylated exopolysaccharide (DFcP) on the infant fecal microflora were evaluated in vitro in this study. The results of single-strain culture showed that DFcP was selectively utilized by Bifidobacterium longum subsp. infantis ATCC 15697, B. breve ATCC 15700 and Lactobacillus plantarum CGMCC 1.19, and promoted the accumulation of short chain fatty acids (SCFAs). Compared with 2’-fucosylactose (2’-FL), DFcP was utilized more quickly by the fecal microbiota and led to higher levels of SCFA production (34.57 mmol/L, P < 0.05) during in vitro fermentation. The results of single-molecule real-time sequencing (SMRT) showed that DFcP increased the proportion of Firmicutes in the fecal microbiota, up-regulated the relative abundance of Enterococcus, Lactobacillus and Bifidobacterium, which was verified by the results of real-time polymerase chain reaction (PCR). In conclusion, DFcP could be rapidly utilized by the infant fecal microbiota, and significantly regulate its structure and promote the accumulation of the beneficial metabolite SCFAs. This study reveals the potential of DFcP as new prebiotics, which provides a theoretical basis for further development of novel fucosylated oligosaccharide resources.

Key words: fucosylated oligosaccharides; infant gut microflora; Bifidobacterium; short-chain fatty acids; third-generation full-length sequencing