食品科学 ›› 2024, Vol. 45 ›› Issue (11): 293-300.doi: 10.7506/spkx1002-6630-20230619-149

• 专题论述 • 上一篇    下一篇

基于荧光共振能量转移效应的荧光传感器在真菌毒素检测中的应用

卢美君,王田林,李天歌,乔明武,马燕,黄现青,范家霖,宋莲军   

  1. (1.河南农业大学食品科学技术学院,河南?郑州 450002;2.河南省科学院同位素研究所有限责任公司,河南?郑州 450002)
  • 出版日期:2024-06-15 发布日期:2024-06-13
  • 基金资助:
    河南省自然科学基金青年基金项目(232300421259);2022年河南省研究生联合培养基地项目(YJS2022JD16); 河南省高校科技创新团队支持计划资助项目(23IRTSTHN023);河南省科学院科技开放合作项目(220904004)

A Review on the Application of Fluorescence Sensors Based on Fluorescence Resonance Energy Transfer Effect for Mycotoxins Detection

LU Meijun, WANG Tianlin, LI Tiange, QIAO Mingwu, MA Yan, HUANG Xianqing, FAN Jialin, SONG Lianjun   

  1. (1. School of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, China; 2. Institute of Isotope Research, Henan Academy of Sciences, Zhengzhou 450002, China)
  • Online:2024-06-15 Published:2024-06-13

摘要: 真菌毒素对人和动物具有剧毒和致癌性,且预防和控制其对食品造成的污染较为困难,因此人们对真菌毒素的关注度越来越高。检测食品中的真菌毒素十分必要,传统检测方法的检测结果准确、可靠,但所需设备昂贵、检测时间长,不符合快速检测真菌毒素的要求。因此,需要开发出快速、灵敏、准确且经济的真菌毒素检测方法。基于荧光共振能量转移(fluorescence resonance energy transfer,FRET)效应的荧光传感器由于操作简单、反应速度快、结果可靠且成本低而广泛应用于检测行业。本文主要介绍了基于FRET效应荧光传感器的检测机制,综述了该传感器在真菌毒素检测中的应用情况,提出了目前荧光传感器仍存在的问题并对其未来的发展趋势进行了展望,以期为新型荧光传感器的设计及真菌毒素检测时灵敏度的优化提供参考。

关键词: 真菌毒素;荧光;传感器;荧光共振能量转移;检测

Abstract: Mycotoxins are highly toxic and carcinogenic to humans and animals, and mycotoxin pollution in foods is difficult to prevent and control, which has sparked increasing concern. Therefore, it is necessary to detect mycotoxins in foods. Traditional mycotoxin detection methods, although accurate and reliable, require expensive equipment and long detection time, which do not meet the requirements of the rapid detection of mycotoxins. Thus, it is necessary to develop fast, sensitive, accurate, and economical methods for detecting mycotoxins. Fluorescent sensors based on the fluorescence resonance energy transfer (FRET) effect are widely used in the detection industry because of their simple operation, fast reaction speed, reliable results and low cost. This article mainly introduces the detection mechanism of fluorescence sensors based on the FRET effect, summarizes their applications in mycotoxin detection, and proposes existing problems and an outlook for future development trends, in order to provide a reference for the design of novel fluorescence sensors and the optimization of the sensitivity of mycotoxin detection.

Key words: mycotoxins; fluorescence; sensors; fluorescence resonance energy transfer; detection

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