砷脂促进秀丽隐杆线虫衰老的毒性效应及其分子机制

doi:10.7506/spkx1002-6630-20231226-227

食品科学 ›› 2024, Vol. 45 ›› Issue (23): 131-139.doi: 10.7506/spkx1002-6630-20231226-227

• 营养卫生 • 上一篇

砷脂促进秀丽隐杆线虫衰老的毒性效应及其分子机制

尹欢，宋兵兵，黎梓玉，汪卓，赵巧丽，李瑞，陈建平，钟赛意

（1.广东海洋大学食品科技学院，广东省水产品加工与安全重点实验室，广东省海洋生物制品工程实验室，广东省海洋食品工程技术研究中心，广东省亚热带果蔬加工科技创新中心，广东湛江 524088；2.广东海洋大学深圳研究院，广东深圳 518108）

发布日期:2024-12-06
基金资助:
国家自然科学基金面上项目（31972163）；广东省高校重点领域专项（2023ZDZX2025）；广东省高校科技创新团队项目（2021KCXTD021）

Toxic Effect and Molecular Mechanism of Arsenolipids on Promoting Aging in Caenorhabditis elegans

YIN Huan, SONG Bingbing, LI Ziyu, WANG Zhuo, ZHAO Qiaoli, LI Rui, CHEN Jianping, ZHONG Saiyi

(1. Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Marine Food, Guangdong Sub-tropical Fruit and Vegetable Processing Technology Innovation Center, College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China; 2. Shenzhen Institute of Guangdong Ocean University, Shenzhen 518108, China)

Published:2024-12-06

摘要/Abstract

摘要： 以秀丽隐杆线虫（Caenorhabditis elegans）为模型，研究AsHC 332和AsHC 346等常见于海产品中两种不同分子质量砷脂对线虫衰老和寿命相关指标的影响，并通过测定对线虫DAF-16蛋白表达情况、衰老突变株寿命和应激相关基因的影响分析其促衰老的可能机制。结果表明，砷脂以浓度依赖性的方式显著降低了线虫体长、体宽、后代数目和头部摆动频率（P＜0.05）；降低了线虫对热应激（P＜0.000 1）和紫外应激抗逆性（P＜0.05）；显著缩短了线虫寿命（P＜0.01）；增加了线虫脂褐素积累（P＜0.001）；上调了daf-16、sod-3、ctl-1、gst-4和hsp-16.2基因相对表达，对线虫DAF-16蛋白核定位促进作用不显著，对daf-16(mu86)突变株寿命无影响。研究表明AsHC 332和AsHC 346对线虫衰老的毒性效应相同，可能依赖于DAF-16转录因子的调节，从而缩短线虫寿命。

关键词: 砷脂；有机砷；毒性；衰老；分子机制；秀丽隐杆线虫

Abstract: In this study, we explored the effect of two arsenolipids with different molecular masses, AsHC 332 and AsHC 346, which are commonly found in seafood, on aging and lifespan-related indicators in the model organism Caenorhabditis elegans. The possible mechanism by which the arsenolipids promote aging was analyzed by measuring the expression of DAF-16 protein in the nematode, the longevity of aging mutants and stress-related genes. The results showed that AsHCs significantly reduced the body length, body width, brood size and head thrash frequency of C. elegans in a dose-dependent manner (P < 0.05). Moreover, the resistance to heat stress (P < 0.000 1) and ultraviolet stress (P < 0.05) was significantly reduced by AsHCs. The lifespan of C. elegans was significantly shortened (P < 0.01). The accumulation of lipofuscin was increased (P < 0.001). The relative expression of the daf-16, sod-3, ctl-1, gst-4 and hsp-16.2 genes was up-regulated, while the nuclear localization of DAF-16 was not significantly promoted, and there was no effect on the lifespan of the daf-16(mu86) mutant. AsHC 332 and AsHC 346 had the same toxic effects on nematode aging, which may shorten the lifespan of C. elegans by regulating the transcription factor DAF-16.

Key words: arsenolipids; organic arsenic; toxicity; aging; molecular mechanism; Caenorhabditis elegans

中图分类号:

TS254.7

尹欢，宋兵兵，黎梓玉，汪卓，赵巧丽，李瑞，陈建平，钟赛意. 砷脂促进秀丽隐杆线虫衰老的毒性效应及其分子机制[J]. 食品科学, 2024, 45(23): 131-139.

YIN Huan, SONG Bingbing, LI Ziyu, WANG Zhuo, ZHAO Qiaoli, LI Rui, CHEN Jianping, ZHONG Saiyi. Toxic Effect and Molecular Mechanism of Arsenolipids on Promoting Aging in Caenorhabditis elegans[J]. FOOD SCIENCE, 2024, 45(23): 131-139.

[1]	尹欢宋兵兵黎梓玉汪卓赵巧丽李瑞陈建平钟赛意. 砷脂促进秀丽隐杆线虫衰老的毒性效应及其分子机制[J]. 食品科学, 0, (): 0-0.
[2]	裘奕星刘鑫宋羽梵丁玉庭徐霞. 基于磁性MOF纳米酶的比色适配体传感器一步法检测鱼肉中组胺[J]. 食品科学, 0, (): 0-0.
[3]	李增蔚，陆晶红，余梦海，吴杰，杨敏，梁艳，邓尚贵，袁鹏翔. 利用生物阻抗无损测定养殖活大黄鱼组分[J]. 食品科学, 2024, 45(10): 281-289.
[4]	赵亚菲姚远钟克利孙小飞李学鹏汤立军励建荣. 萘酚-苯并噻唑衍生物的合成及其琼脂糖凝胶对三文鱼新鲜度双通道无损检测[J]. 食品科学, 0, (): 0-0.
[5]	孙瑞雪方禹文陈冬妍陈全胜陈晓梅. 基于石墨烯泡沫的电增强固相微萃取-高效液相色谱法测定鱼肉中三种磺胺类的残留[J]. 食品科学, 0, (): 0-0.
[6]	孙瑞雪，方禹文，陈冬妍，陈全胜，陈晓梅. 基于石墨烯泡沫的电增强固相微萃取-高效液相色谱法测定鱼肉中3 种磺胺类的残留[J]. 食品科学, 2024, 45(5): 265-274.
[7]	占可，陈季旺，徐言，倪杨帆，刘言，邹圣碧. 基于近红外光谱特征的冷冻小龙虾鲜度快速检测方法[J]. 食品科学, 2024, 45(2): 299-307.
[8]	占可陈季旺徐言倪杨帆刘言邹圣碧. 基于近红外光谱特征的冷冻小龙虾鲜度快速检测方法[J]. 食品科学, 0, (): 0-0.
[9]	金文刚，刘俊霞，孙海燕，何琳琳，裴金金，程虎，姜鹏飞. 基于GC-IMS结合化学计量学鉴别大鲵油掺伪不同比例花生油挥发性有机物特征[J]. 食品科学, 2023, 44(10): 368-376.
[10]	陈佳佳，钟映雄，周雪巍，李瑞，陈建平，刘晓菲，贾学静，宋兵兵，钟赛意. 海洋生物中砷脂形态分析及毒性评价研究进展[J]. 食品科学, 2023, 44(1): 277-284.
[11]	郑瑞生王巧燕张冰泉李丹萍邹菊琴郑宗平. 9种近海鱼类重金属污染状况及食用安全性评价[J]. 食品科学, 0, (): 0-0.
[12]	陈荣锋陈凤明潘裕添张国广张峰. 基于QuEChERS-液相色谱-质谱联用技术检测食用菌中8种烟碱类化合物[J]. , 0, (): 0-0.
[13]	吴烁，张彪，张万利，邵静梅，刘飘雪，生威. 荧光微球免疫层析方法定量检测水产品中4 种硝基呋喃代谢物[J]. 食品科学, 2021, 42(20): 299-304.
[14]	张星晖，李婷婷，励建荣，于昕睿，梅佳林，徐宇辰，谢晶，王轰，王明丽，申照华，郭晓华，劳敏军，周小敏，于建洋. 溴麝香草酚蓝/玉米醇溶蛋白指示标签对金枪鱼新鲜度的监测[J]. 食品科学, 2021, 42(19): 226-233.
[15]	吴浩周秀雯陈海泉靳保辉颜治赵旭谢丽琪赵燕林光辉. 不同产地三文鱼的稳定同位素指纹特征及原产地溯源[J]. , 0, (): 0-0.

砷脂促进秀丽隐杆线虫衰老的毒性效应及其分子机制

Toxic Effect and Molecular Mechanism of Arsenolipids on Promoting Aging in Caenorhabditis elegans

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价