溴麝香草酚蓝/玉米醇溶蛋白指示标签对金枪鱼新鲜度的监测

doi:10.7506/spkx1002-6630-20201111-110

食品科学 ›› 2021, Vol. 42 ›› Issue (19): 226-233.doi: 10.7506/spkx1002-6630-20201111-110

• 包装贮运 • 上一篇

溴麝香草酚蓝/玉米醇溶蛋白指示标签对金枪鱼新鲜度的监测

张星晖，李婷婷，励建荣，于昕睿，梅佳林，徐宇辰，谢晶，王轰，王明丽，申照华，郭晓华，劳敏军，周小敏，于建洋

（1.渤海大学食品科学与工程学院，生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心，辽宁锦州 121013；2.大连民族大学生命科学学院，辽宁大连 116600；3.上海海洋大学食品学院，上海 201306；4.蓬莱京鲁渔业有限公司，山东烟台 265600；5.山东美佳集团有限公司，山东日照 276800；6.浙江兴业集团有限公司，浙江舟山 316120；7.荣城泰祥食品股份有限公司，农业农村部冷冻调理海洋食品加工重点实验室，山东威海 264309）

发布日期:2021-11-12
基金资助:
“十三五”国家重点研发计划重点专项（2018YFD0400601；2019YFD0901702）

Monitoring the Freshness of Tuna with Bromothymol Blue/Zein Indicator Label

ZHANG Xinghui, LI Tingting, LI Jianrong, YU Xinrui, MEI Jialin, XU Yuchen, XIE Jing, WANG Hong, WANG Mingli, SHEN Zhaohua, GUO Xiaohua, LAO Minjun, ZHOU Xiaomin, YU Jianyang

(1. National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, College of Food Science and Engineering, Bohai University, Jinzhou 121013, China; 2. College of Life Science, Dalian Minzu University, Dalian 116600, China; 3. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; 4. Penglai Jinglu Fishery Co., Ltd., Yantai 265600, China; 5. Shandong Meijia Group Co., Ltd., Rizhao 276800, China; 6. Zhejiang Xingye Industrial Group Co., Ltd., Zhoushan 316120, China; 7. Key Laboratory of Frozen Prepared Marine Foods Processing, Ministry of Agriculture and Rural Affairs, Rongcheng Taixiang Food Co., Ltd., Weihai 264309, China)

Published:2021-11-12

摘要/Abstract

摘要： 近年来，智能检测食品新鲜度的比色膜受到越来越多的关注。本实验利用静电纺丝技术研制出一种溴麝香草酚蓝（bromothymol blue，BTB）/玉米醇溶蛋白（zein，ZN）新型智能比色标签，用于金枪鱼鱼片的新鲜度监测。BTB在pH值为5～12时的明显颜色变化赋予了指示标签灵敏的pH值传感效果。通过场发射扫描电子显微镜、傅里叶变换红外光谱、X射线衍射、差示扫描量热仪对不同BTB添加量的BTB/ZN指示标签的形貌和结构进行表征。再将BTB/ZN指示标签应用于金枪鱼鱼片新鲜度的指示验证实验，对指示标签的指示效果进行验证。结果表明，BTB/ZN指示标签的微观结构良好，BTB可以很好地与ZN结合在一起，并且不破坏二者的结构；质量分数4% BTB的BTB/ZN指示标签的指示效果最佳，能灵敏地反映金枪鱼的新鲜度变化。指示标签的色度和金枪鱼鱼肉的总挥发性盐基氮含量之间存在显著相关性，这证明BTB/ZN指示标签能有效监测金枪鱼鱼片的新鲜度。

关键词: 静电纺丝；金枪鱼；玉米醇溶蛋白；溴麝香草酚蓝；指示标签

Abstract: In recent years, more and more attention has been paid to smart colorimetric membranes for detection of food freshness. In this work, a new smart colorimetric label made from bromothymol blue (BTB) and zein (ZN) was developed by electrospinning to monitor the freshness of tuna fillets. The color of BTB changed obviously in the pH range of 5–12, thus making the indicator label a sensitive pH sensor. The morphology and structure of BTB/ZN nanofiber indicator labels prepared with different amounts of BTB were characterized by field emission scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and differential scanning calorimetry (DSC). Then, the performance of the indicator label prepared under the optimized condition was evaluated by applying it to real samples. The results showed that the microstructure of BTB/ZN nanofiber membranes was good, and BTB was well combined with ZN without destroying their structures. The indicator label with 4% BTB was the most sensitive to reflect freshness changes of tuna. There was a significant correlation between the color of the indicator label and the total volatile basic nitrogen (TVB-N) content of tuna meat, which proves that BTB/ZN indicator label can effectively monitor the freshness of tuna fillets.

Key words: electrospinning; tuna; zein; bromothymol blue; indicator label

中图分类号:

TS254.7

张星晖，李婷婷，励建荣，于昕睿，梅佳林，徐宇辰，谢晶，王轰，王明丽，申照华，郭晓华，劳敏军，周小敏，于建洋. 溴麝香草酚蓝/玉米醇溶蛋白指示标签对金枪鱼新鲜度的监测[J]. 食品科学, 2021, 42(19): 226-233.

ZHANG Xinghui, LI Tingting, LI Jianrong, YU Xinrui, MEI Jialin, XU Yuchen, XIE Jing, WANG Hong, WANG Mingli, SHEN Zhaohua, GUO Xiaohua, LAO Minjun, ZHOU Xiaomin, YU Jianyang. Monitoring the Freshness of Tuna with Bromothymol Blue/Zein Indicator Label[J]. FOOD SCIENCE, 2021, 42(19): 226-233.

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溴麝香草酚蓝/玉米醇溶蛋白指示标签对金枪鱼新鲜度的监测

Monitoring the Freshness of Tuna with Bromothymol Blue/Zein Indicator Label

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