食品科学 ›› 1999, Vol. 20 ›› Issue (7): 18-21.

• 基础研究 • 上一篇    下一篇

胰蛋白酶水解鲨鱼鳍软骨提取粘多糖的研究

 肖凯军, 郭祀远, 李琳, 陈健, 黎旭茹   

  1. 华南理工大学食品与生物工程学院轻化工所
  • 出版日期:1999-07-15 发布日期:2011-12-06

 XIAO  Kai-Jun, GUO  Si-Yuan, LI  Lin, CHEN  Jian, LI  Xu-Ru   

  • Online:1999-07-15 Published:2011-12-06

摘要: 对胰蛋白酶水解鲨鱼鳍软骨提取粘多糖进行了较系统的研究。研究了预加热处理对酶水解鲨鱼鳍软骨提取粘多糖的促进作用,考察了时间、温度、pH和酶添加量对酶解鲨鱼鳍软骨提取粘多糖和硫酸含量的影响,在单因素实验的基础上,通过正交实验得出胰蛋白酶酶解鲨鱼鳍软骨的最佳工艺条件,比较了三氯乙酸、氯仿-丁醇以及氯仿-戊醇等蛋白沉淀剂除杂蛋白的效果,并对鲨鱼鳍软骨粘多糖进行了初步提纯。

关键词: 胰蛋白, 粘多糖, 鲨鱼鳍软, 水解, 提取

Abstract: Extraction of Mucopolysaccharides from shark fin cartilage by digested with trypsinase was studied.The increase of heating pretreatment could improve the hydrolysis of shark fin cartilage and increase the content of mucopolysaccharides and sulfate in the extraction in the range 70℃to 90℃.The effects of temperature, pH, enzymatic addition and time of hydrolysis of shark fin cartilage with trypsinase were investigated and the optimum hydrolysis condition was as follows:with 3% of enzymatic addition at 50℃and pH 8.0 for 26h. Among chloroform-butyl alcohol (9:1),chloroform-Pentanoic alconol (9: 1) and trichloroacetic acid,trichloroacetic acid was the best protein precipitant for isolation of shark fin cartilage mucopolysaccharides.

Key words: Trypsinase Mucopolysaccharides Shark fin cartilage Hydrolysis Extraction