食品科学 ›› 2000, Vol. 21 ›› Issue (12): 41-43.

• 基础研究 • 上一篇    下一篇

产β-呋喃果糖苷酶黑曲霉的固定化研究

 生庆海, 程建军, 骆承痒   

  1. 东北农业大学食品学院
  • 出版日期:2000-12-15 发布日期:2011-12-05

Study on Immoblilzation of FTS by Sodium Alginate

 SHENG  Qing-Hai, CHENG  Jian-Jun, LUO  Cheng-Yang   

  • Online:2000-12-15 Published:2011-12-05

摘要: 对β-呋喃果糖苷酶的黑曲霉采用海藻酸钠包埋的方法,直接制成固定化菌丝生产FOS(Fructooligosacchavides)的试验,采用两因素二次饱和D-最优设计方案,对破碎菌体与海藻酸钠溶液的混合最佳比例以及海藻酸钠溶液的最佳浓度进行研究,并计算其半衰期。结果为:菌体与海藻酸钠溶液混合比例定为1:1,海藻酸钠溶液浓度定为2%,半衰期为273.5h。

关键词: &beta, -呋喃果糖苷酶; 海藻酸钠; 固定化; 半衰期;

Abstract: This article studied on the immobilization material 杝odium alginate.By using D-optimization saturated experiment design,the ratio of the entrapment of whole cell with sodium alginate was 1:1 (whole cell:2% sodium alginate).The activity of the immobilized enzym was 851U/g (84.5% of the crude enzyme) under the specified conditions The half-life of the immobilized-enzyme was 273.5h.

Key words: &beta, -FTS Sodium alginate Immobilization Half-time T;