食品科学 ›› 2000, Vol. 21 ›› Issue (8): 13-15.

• 基础研究 • 上一篇    下一篇

茶叶多糖的纯化及组分研究

许新德, 高荫榆, 陈才水, 刘梅森   

  1. 南昌大学生命科学与食品工程学院
  • 出版日期:2000-08-15 发布日期:2011-12-01

Study on Purification and Composition of Crude Tea Polysaccharide

 XU  Xin-De, GAO  Yin-Yu, CHEN  Cai-Shui, LIU  Mei-Sen   

  • Online:2000-08-15 Published:2011-12-01

摘要: 将粗老绿茶多糖过DEAE-纤维素柱,得一中性多糖及TPS Ⅰ、 TPS Ⅱ、 TPS Ⅲ三个酸性多糖。经糖腈乙酰化处理后进行气相色谱分析知中性糖由Ara.、Xyl.、Man.、Glu.、Gal.组成,其比例为1.04:2.98:5、99:2.00:1.00;TPSⅠ由Ara.、Xyl.、Glu.、Cal组成,其比例为257:094:1.77:1.00;TPSⅡ由Ara.、Xyl.、Glu.、Gal组成,其比例为2.57:0.94:1.77:1.00;TPSⅢ由Rha.、Ara.、Xyl.、Gal.组成,其比例为5:2:1:7。这些为探讨茶多糖的抑制巧克力起霜机理提供了依据。

关键词: 茶叶多糖, 气相色谱, 抑霜

Abstract: The crude tea polysaccharide (CTPS) was eluted from DEAE-cellulose column by NaCl graded elution. The results showed that CTPS was composed of four components-one neutral polysaccharide and three acid polysaccharides (TPS Ⅰ. TPS Ⅱ、TPS Ⅲ) .After converting the hydrolyzed polysaccharides into acetylated aldononitriles by means of gas chromatography, the chemical components and the ratio were showed as follows: the neutral polysaccharides: D-Ara.: D- Ⅰ:D-Rha. :D-Man:D-Glu.:D-Gal.=0.48:0.56:1 .33. 1.00; TPS Ⅲ:D-Rha.: D-Ara. :D-Xyl.:D-Gal.=5:2:1.7.All these could be used for further study into the mechanism of antibloom of chocolate.

Key words: Tea Ploysaccharide Gas Chromatography Antibloom