食品科学 ›› 2001, Vol. 22 ›› Issue (10): 35-37.

• 基础研究 • 上一篇    下一篇

固定化醋酸杆菌细胞特性的研究

 罗自生,  席玙芳,  张惟广, 曾繁坤,  吴永娴   

  1. 浙江大学食品科技系; 西南农业大学食品科学系
  • 出版日期:2001-10-15 发布日期:2012-02-15

Study on Characteristics of Immobilized Acetobacter Cells
 

 LUO  Zi-Sheng,   Xi-Yu-Fang,   Zhang-Wei-Guang, ZENG  Fan-Kun,   Wu-Yong-Xian   

  • Online:2001-10-15 Published:2012-02-15

摘要: 研究了用于柑桔汁脱苦的固定化醋酸杆菌细胞的特性,研究表明:固定化细胞脱苦最佳供氧为:摇床转速160r/min,最适pH为4.5,最适温度为25℃;而游离细胞脱苦的相应条件分别为160r/min,pH5.5,25℃。低浓度NaCl对固定化细胞和游离细胞的脱苦都有强烈的抑制作用;固定化细胞在柠碱浓度达到30mg/kg后,柠碱降解速度趋于饱和,而游离细胞的柠碱浓度则为35mg/kg;固定化细胞的热稳定性比游离细胞好。

关键词: 醋酸杆菌, 柠碱, 脱苦, 固定化细胞

Abstract:  Characteristics of immobilized acetobacter cells for limonin debittering debittering in citrus juices were investigated.The immobilized cells for optimal debittering showed that the shaking flask should be running 106r/min,pH 4.5,25℃, while the shaking tlask of the relative debittering of the free cells were running at 160r/min,pH5.5.25℃.Low concentration of sodium chloride inhibited debittering of immobilized and free cells.Debittering rate came to a saturation with limonin concentration of 30 mg/kg for the immobilized cells while for the free cells with the concentration of 35mg/kg.Heat stability of immobilized cells worked better than that of free cells.

Key words: Acetobacter Limonin Debittering Immobilized cells