食品科学 ›› 2001, Vol. 22 ›› Issue (12): 19-22.

• 基础研究 •    下一篇

两种植物蛋白酶对珍珠壳角蛋白降解效果的观察

 宋慧春,  范雁, 王雪峰   

  1. 苏州大学生命科学学院
  • 出版日期:2001-12-15 发布日期:2012-02-14

Observation Study on Conchiolin Enzymolysis Effects of The Two Kinds of Plant Protease

 SONG  Hui-Chun,   Fan-Yan, WANG  Xue-Feng   

  • Online:2001-12-15 Published:2012-02-14

摘要: 采用菠萝蛋白酶和木瓜蛋白酶制剂对从珍珠贝壳珍珠层中提取的珍珠壳角蛋白进行酶促降解实验,用紫外分光光度法检测降解产物小肽及酪氨酸在275nm波长的紫外吸收值,以观察分析这两种植物蛋白酶对壳角蛋白的降解效果,探讨其酶促降解的条件实验。结果表明:两种植物蛋白酶对壳角蛋白都有一定的降解作用,菠萝蛋白酶降解效果优于木瓜蛋白酶,它在底物浓度小于0.2%时,与酶解产物呈线性关系,酶浓度一般宜保持在100μg/ml以下,酶促反应时间以10h为佳。

关键词: 壳角蛋白, 珍珠贝壳, 菠萝蛋白酶, 木瓜蛋白酶

Abstract: Enzymolysis test about the conchiolin extracted from the shell nacre of pearls by using pineapple protease and papain was made to examine the UV absorbance of peptide and tyrosine at the wavelength of 275 nm with ultraviolet spectrometry.After observing and analyzing conchiolin enzymolysis effect of the two kinds of plant protease,we probed into the conditions of its enzymolysis.The conclusion showed.The two kinds of plant protease have had particular enzymolysis effects and the enzymolysis effect of pineapple protease was higher than the papain’s.It appeared a line relation ship with enzymolysis products when the substrate concentration was less than 0.2%.It was optimal when the enzyme concentration was kept under 100μg/ml and enzymolysis time about 10 hours.

Key words: Conchiolin Pearl shell Pineapple protease Papain