食品科学 ›› 2002, Vol. 23 ›› Issue (6): 80-84.

• 工艺技术 • 上一篇    下一篇

富锗酵母研究

 王凤玲, 邵晓芬, 王银华   

  1. 天津商学院食品系
  • 出版日期:2002-06-15 发布日期:2012-02-13

Study on Cerevisiea Enriched by Gera nium

 WANG  Feng-Ling, SHAO  Xiao-Fen, WANG  Yin-Hua   

  • Online:2002-06-15 Published:2012-02-13

摘要: 以二氧化锗为锗源,确定葡萄酒酵母和啤酒酵母对锗的生物富集的工艺条件。结果表明,葡萄酒酵母对锗具有良好的生物富集作用,在锗的加入量为10×10-6的10°Brix的麦芽汁培养基中进行葡萄酒酵母的发酵培养,所得酵母对锗的富集量达98.2μg/g,有机化率达94%;Ge4+在磷酸的乙醇溶液中参与桑色素形成稳定的荧光络合物,测定波长λEx=426nm,λEx=496nm。

关键词: 锗, 葡萄酒酵母, 生物富集, 荧光值

Abstract: In this paper,research was made to determine the conditions under which Geraniumwas enriched in sac-charomyces.The resultshowsedthatthe Saccharomyces cerevisiae could do wellinthe enrichmentofGeranium.Inthe malt mediumof 10°Brix into which a quantity of 10×10 -6 Geraniumwas added,the yeast was thu s cultured for about 20h,whentheyeastcells almostscop pedgrowingwhichcouldbeseenfromb iomass.Itturnedouttobethattheen riched quantity of Geraiumwas 98.2μg /g,and the organically changed rate 94%.we also found that in Phosphoric acid -ethylalcoholsolutionGe 4+ andmorinwouldproduceastablecomp lexofstrongfluoranthene,forwhic hhedetectivelimit of Geraniumwas 0.001μg /g,andthe relative standard devia tion4.26%.

Key words: Geranium , Saccharomyces Cerevisiea , Biological enrichment , Spectrofluorometry