食品科学 ›› 2002, Vol. 23 ›› Issue (7): 25-27.

• 基础研究 •    下一篇

没食子单宁与蛋白质、磷酯和糖的反应研究

 何强, 石碧, 姚开, 贾冬英   

  1. 四川大学轻纺与食品工程学院
  • 出版日期:2002-07-15 发布日期:2011-12-31

Interaction Study on Gallotannins with Proteins,Phospholipids and Sugars

 HE  Qiang, SHI  Bi, YAO  Kai, JIA  Dong-Ying   

  • Online:2002-07-15 Published:2011-12-31

摘要: 本文定量研究了没食子单宁(β-1,2,6-三没食子酰基-D-葡萄糖TGG和β-1,2,3,4,6-五没食子酰基-D-葡萄糖PGG)与多种蛋白质、磷酯和糖的反应。结果表明没食子单宁与蛋白质(组蛋白、牛血清清蛋白、酪蛋白和明胶)及磷酯(卵磷脂、脑磷酯和神经磷酯)有较强的结合作用,而与糖的结合较弱,PGG比TGG具有更强的与生物质(蛋白质、磷酯和糖)结合的能力。实验表明,蛋白质-没食子单宁反应和磷酯-没食子单宁反应是氢键和疏水键协同作用的结果,而在糖没食子单宁反应中氢键是主要的作用方式。

关键词: 没食子单宁, 生物质, 结合, 抗营养作用

Abstract: The binding of gallotannins(β-1,2,6-tri -O -galloyl -D -glucose TGGandβ-1,2,3,4,6-penta -O -galloyl -D -glucose PGG)on typical proteins,phospholipids and sugars was examined quantitativ e-ly.The results indicated that gallo tannins bound more on proteins(histone,bovine serumalbumin,case inand gelatin)andphospholipids(L -α-lecithin,L -α-cephalin andSphingomyelin)than onsugars,andthat PGGhadstron ger binding affinity on proteins,phosp holipids and sugars than TGG.It also showed that protein -gallotannin an d phos-pholipid -gallotannininteactions were the results of cooperative effe cts of hydrogen and hydrophobic bond s,and that hydrogenbond was the predominant effect in the interactions betweengallotannins andsugars.

Key words: Gallotannins , Biomass , Binding , Antin utritional effects