花生壳中木犀草素等抑菌活性成分的提取、纯化与研究

食品科学 ›› 2003, Vol. 24 ›› Issue (5): 84-88.

花生壳中木犀草素等抑菌活性成分的提取、纯化与研究

陈春涛, 马庆一, 高玉美, 孙红艳

郑州轻工业学院食品与生物工程系

出版日期:2003-05-15 发布日期:2011-12-13

Study on Extraction, Purification and Antimicrobial Activity Investigation of Luteolin and Other Components in Peanut Hulls

CHEN Chun-Tao, MA Qing-Yi, GAO Yu-Mei, SUN Hong-Yan

Online:2003-05-15 Published:2011-12-13

摘要/Abstract

摘要： 本实验以甲醇粗提、溶剂梯度萃取、配合抑菌活性跟踪检测,得到了抑菌活性较强、富含黄酮类的花生壳乙酸乙酯组分,并用酸碱沉淀、柱层析等法纯化得到三种化合物(1#,2#,3#)。其中2#从紫外光谱法确认为木犀草素,得率为0.53%。它对金黄色葡萄球菌和枯草杆菌有一定的抑制效果。实验还发现3#对金黄色葡萄球菌、酿酒酵母有一定的抑菌活性;1#则具有更广的抑菌谱,它对金黄色葡萄球菌、酿酒酵母、枯草杆菌和青霉均有较强的抑制作用。它的紫外图谱具有二氢黄酮的特点,其结构有待进一步实验认证。

关键词: 木犀草素, 柱层析法, 滤纸片法, 抑菌活性

Abstract: In this experiment the flavonoids in peanut hulls with strong antimicrobial activity were found in ethyl acetatecomponent by methanol extraction and then successive polarity-increase solvent extraction to monitor the antimicrobial effect.Through base-acid precipitation, and TLC purification three compounds (1# 2# 3#) were obtained and 2# was confirmed by UVchemical structure analysis as Luteolin (the yield was 0.53 %) to have antimicrobial activities against Staphylococcus Aurous andBacillus subtilis , while 3# to have activities against Staphylococcus Aurous and Saccharomyces. 1# should even broader antimicrobialeffects on Staphylococcus Aurous,Saccharomyces , Bacillus subtilis,and Penicilliam. According to UV, 1# might belong toflavanones, and need to be determined further.

Key words: Luteolin, TLC purification, antimicrobial activity

陈春涛, 马庆一, 高玉美, 孙红艳. 花生壳中木犀草素等抑菌活性成分的提取、纯化与研究[J]. 食品科学, 2003, 24(5): 84-88.

CHEN Chun-Tao, MA Qing-Yi, GAO Yu-Mei, SUN Hong-Yan. Study on Extraction, Purification and Antimicrobial Activity Investigation of Luteolin and Other Components in Peanut Hulls[J]. FOOD SCIENCE, 2003, 24(5): 84-88.

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