关键词: 灰树花, 多糖, PGF-1, 羟基自由基, 抗氧化

Abstract: The scavenging effect of PGF-1 (one kind of homogeneous polysaccharide from Grifola frondosa ) on hydroxylradicals (•OH) in deoxyribose-iron system was studied. To study the protective activities of PGF-1 against oxidation damagecaused by •OH in vitro, the content of malonaldehyed (MDA) in mice liver juice and mice liver mitochondria, the swellingextent of mice liver mitochondria, the mice liver mitochondria membrane fluidity and the hemolysis of rat red blood cell were assayed.Results showed that PGF-1 with obvious inhibitory effect on free radical of •OH. PGF-1 could decrease the formation of MDAinduced by •OH and inhibit the swelling extent of mice liver mitochondria. It also had protective activity on the mice livermitochondria membrane fluidity and inhibitory effect on the hemolysis of rat red blood cell induced by •OH. It suggested thatPGF-1 showed strong inhibitory effect on •OH in vitro.

Key words: grifola frondosa, polysaccharide, PGF-1, hydroxyl radicals, antioxidation