食品科学 ›› 2003, Vol. 24 ›› Issue (7): 89-92.

• 工艺技术 • 上一篇    下一篇

Xanthomonas ampelina发酵培养基组成的优化

 宋丽萍, 陈庆森, 闫亚丽, 刘健   

  1. 天津商学院食品与生物工程系
  • 出版日期:2003-07-15 发布日期:2011-12-13

 SONG  Li-Ping, CHEN  Qing-Sen, YAN  Ya-Li, LIU  Jian   

  • Online:2003-07-15 Published:2011-12-13

摘要: 本文采用正交实验的方法优化了Xanthomonas ampelina TS206发酵培养基的配方,从而达到了提高冰核活性细菌发酵所得的生物量和细菌的冰核活性的目的。实验结果表明较优化的培养基配方为:牛肉膏3.0g/L、蛋白胨5.0g/L、甘油25.0g/L、蔗糖20.0g/L、山梨醇2.0g/L、酵母粉20.0g/L、玉米浆20.0g/L、K2HPO4 1.5g/L、pH=7.0;最终发酵液的生物量达到8.2g/L(干重),冻滴率达到81%。

关键词: 正交实验, 发酵培养基, 冰核活性细菌, 生物量, 冰核活性

Abstract: Abstact:The fermentation of Xanthomonas ampelinaTS206 was studied by orthogonal experiment in this paper. The purposewas to deduce the optimal formulation of the culture medium for improving ice nucleation activity and the biomass of icenucleation-active bacteria (X.ampelinaTS206) in the fermentation process. The results showed that the improved formulation offermentative culture medium was: extract of beef 3.0g/L, peptone 5.0g/L, glycerol 25.0g/L, sucrose 20.0 g/L, sorbitol 2.0 g/L,yeast powder 20.0 g/L, corn steep liquor 20.0g/L and K2HPO4=1.5 g/L with pH=7.0. The dry weight of the bacteria producedthis way was 8.2g/L and the percentage of drop-freezing was 81%.

Key words: orthogonal experiment, culture medium, ice nucleation-active bacteria, biomass, ice nucleation activity