食品科学 ›› 2004, Vol. 25 ›› Issue (1): 139-141.

• 分析检测 • 上一篇    下一篇

水产品中单核细胞增生李斯特氏菌检验研究

 于兵, 麻丽丹, 张勇   

  1. 丹东出入境检验检疫局
  • 出版日期:2004-01-15 发布日期:2011-10-24

Studying the Detection of Listeria Monocytogenes in Aquatic Product

 YU  Bing, MA  Li-Dan, ZHANG  Yong   

  1. Dandong Entry-exit Inspection and Quarantine Bureau
  • Online:2004-01-15 Published:2011-10-24

摘要: 本文是对水产品中单核细胞增生李斯特氏菌常规检验的研究。结合国家标准、行业标准和FDA《细菌学检验手册》第八版中所述方法进行检验。自朝鲜进境的水产品23类、255批中检出李斯特氏菌7株。结果表明,由于食品加工过程对微生物损害极大,使大多数微生物处于濒死状态,因而加速受损细胞恢复是单核细胞增生李斯特氏菌检验前增菌的关键。在检验过程中最初分离时发现了可疑菌落并就此进行确认和得出结论,而不是延长增菌时间继续分离和鉴定,容易产生假阴性结果。

关键词: 水产品, 单核细胞增生李斯特氏菌, 检验

Abstract: This paper studies the conventional detection of Listeria monocytogenes in aquatic product. Combines nationstandard, trade standard with method described in FDA《Bacteriological Analytical Manual》 8th edition during detection.There are seven Listeria positive in 23 kinds,255 batches of aquatic product imported from North korea. The result shows thatbecause of food processing doing severe harm to microbiology, most of them are in the agonal state, so that stimulating the injuredcell to recover is the key work in the Listeria monocytogenes pre-enrichment procedure during detection. When there are suspectcolonies in the first division, do conforming work and get the conclusion at first, instead of going on enriching, it is easy to getfalse negative result.

Key words: aquatic product, Listeria monocytogenes, detection