葛根总黄酮对运动大鼠脑组织GAT-2基因表达影响的初步研究

食品科学 ›› 2004, Vol. 25 ›› Issue (2): 171-174.

葛根总黄酮对运动大鼠脑组织GAT-2基因表达影响的初步研究

唐量, 熊正英, 张英起

陕西师范大学运动生物学研究所，第四军医大学生物技术中心

出版日期:2004-02-15 发布日期:2011-10-24

Effect of Pueraria Flavonid on GAT-2 Expression in the Brains of Exercised Rats

TANG Liang, XIONG Zheng-Ying, ZHANG Ying-Qi

1.Institute of Sports Biology, Shanxi Normal University;2.Center of Biotechnology,Fourth Military Medical University

Online:2004-02-15 Published:2011-10-24

摘要/Abstract

摘要： 目的:应用基因芯片技术研究葛根总黄酮抗中枢疲劳的分子生物学机制。方法:将SD大鼠随机分为三组:安静对照组(A)、运动组(B)和服药组(C)。B组和C组进行7周的大强度耐力跑台训练,随后抽提各组大鼠脑组织mRNA,与Cy3和Cy5标记的cDNA混合探针杂交,通过芯片扫描仪对其荧光强度进行扫描,并用计算机对结果进行分析。结果:基因表达谱显示,与安静组比较,运动组大鼠脑组织中γ-氨基丁酸转运体2(GAT-2)基因上调表达;与运动组比较,服药组大鼠脑组织GAT-2基因上调表达显著。结论:葛根总黄酮对运动大鼠脑组织GAT-2基因有明显上调作用,而GAT-2基因表达与中枢疲劳有一定关联,可作为易感基因在中枢疲劳机制和抗疲劳功能食品筛选中进行研究。

关键词: 基因芯片, 葛根总黄酮, GAT-2, 运动, 脑组织, 大鼠

Abstract: Aim:By genechip cDNA microarray, the biomolecular mechanism of anti-fatigue of Pueraria Flavonid was studiedin the center nerve system(CNS) of the exercise-training rats. Methods: SD rats were randomly divided into three groups: normalrest group(A), exercise-training group(B) and administered-drug group(C). B group and C group rats were trained with highintensity 7 week by treadmill. The total RNAs were isolated from the brain tissue, and were purified to mRNAs by Oligotex.The purified mRNA were reversely transcribed to cDNA with hybridization probes labeled by fluorescent-dUTP. The genechipwas scanned for the fluorescent signals and showed differences by the computer analysis. Results: The GAT-2 gene was up-regulated expression in the brain of exercise-training rats compared with of rest rats and obviously up-regulated expression in thebrain of administered-drug rats compared with of exercise-training rat. Conclusion: GAT-2 gene expression in CNS of thetraining rats is regulated by Pueraria Flavonid, and its expression has an effect on CNS fatigue and plays an important role in thestudy of CNS fatigue mechanism and screening anti-fatigue functional food as new predisposing gene.

Key words: genechip, Pueraria Flavonid, GAT-2, exercise, brain, rat

唐量, 熊正英, 张英起. 葛根总黄酮对运动大鼠脑组织GAT-2基因表达影响的初步研究[J]. 食品科学, 2004, 25(2): 171-174.

TANG Liang, XIONG Zheng-Ying, ZHANG Ying-Qi. Effect of Pueraria Flavonid on GAT-2 Expression in the Brains of Exercised Rats[J]. FOOD SCIENCE, 2004, 25(2): 171-174.

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