食品科学 ›› 2004, Vol. 25 ›› Issue (9): 74-77.

• 基础研究 • 上一篇    下一篇

菊花脑茎叶抗病原菌活性及其有效成分研究

 纪莉莲, 张强华   

  1. 淮阴工学院生物工程系
  • 出版日期:2004-09-15 发布日期:2011-10-24

Studies on the Anti-pathogenic Activities and Effective Components of the Leaves and Stalks from Chrysanthemum nankingense Hand. Mazz

 JI  Li-Lian, ZHANG  Qiang-Hua   

  1. Department of Bioengineering, Huaiyin Institute of Technology
  • Online:2004-09-15 Published:2011-10-24

摘要: 首次以菊花脑提取物对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌等6种常见的病原细菌及黑曲霉、啤酒酵母等4种病原真菌进行了拮抗试验,结果表明菊花脑提取物A、B和C具有较强的拮抗病原菌的活性,且抗菌活性有明显的量效关系,其对各种供试病原细菌的最低抑菌浓度为0.3%~0.5% (g/ml),对供试病原真菌的MIC值从0.3%~1.0%不等。菊花脑提取物A(挥发油)显示了广谱的抗菌范围,其对供试细菌和供试真菌的抑菌效力相当,但菊花脑提取物B和C(非挥发性成分)对供试细菌的拮抗作用(MIC值为0.3%~0.5%)远大于对供试真菌(MIC值为0.7%~1.0%)的作用。菊花脑中起抗菌作用的主要成分是挥发油、总黄酮、苦味素、野菊花内酯等活性成分。

关键词: 菊花脑, 提取物, 抗菌活性, 病原菌, 最低抗菌浓度

Abstract: The anti-pathogenic activities of Chrysanthemum nankingense Hand. Mazz were evaluated against 6 pathogenicbacteria and 4 pathogenic fungi. Results indicated that the 3 extracts (A, B and C) of Chrysanthemum nankingense Hand. Mazzshowed strong inhibitory effects on these pathogens in a concentration-dependent manner. The extract A (volatile fraction) was evidently active both in inhibiting the bacteria and the fungi with 0.3%~0.5% (g/ml) of MIC values. The extract B and C (non-volatile fraction) exhibited a strong inhibition to the test bacteria at MIC values of 0.3%~0.5%, and a poor inhibition to thesefungi with the MIC of 0.7%~1.0%. Some main active components of Chrysanthemum nankingense Hand. Mazz were isolatedand elucidated as volatile oil, total flavoniod, ailanthin, and lactone.

Key words: Chrysanthemum nankingense Hand. Mazz, extraction, anti-pathogenic activity, pathogens, the minimum inhibitory concentration (MIC)