食品科学 ›› 2005, Vol. 26 ›› Issue (1): 94-97.

• 基础研究 • 上一篇    下一篇

泰山赤灵芝液体培养条件优化及多糖含量测定

 姚良同, 林榕姗, 李娟, 刘敏, 赵倩, 贾乐   

  1. 山东农业大学生命科学学院; 山东农业大学生命科学学院
  • 出版日期:2005-01-15 发布日期:2011-09-19

Assay Study on Optimal Cultivation Conditions to Accumulate the Ganoderma Polysaccharide

 YAO  Liang-Tong, LIN  Rong-Shan, LI  Juan, LIU  Min, ZHAO  Qian, JIA  Le   

  1. College of Life Science,Shandong Agricultural University
  • Online:2005-01-15 Published:2011-09-19

摘要: 本文探讨了泰山赤灵芝(Ganodermalucidum)液体培养中碳源、氮源、pH对灵芝多糖积累的影响。结果表明,培养基最适碳源、氮源、pH值分别为蔗糖、蛋白胨、5.0,其配方组成(质量分数,%)为:KH2PO40.1,MgSO4·7H2O0.05,(NH4)2SO40.2,蛋白胨0.3,蔗糖5,pH5.0,灵芝多糖含量可达11.77%,分别是子实体和孢子粉的2.5和2.2倍。

关键词: 泰山赤灵芝, 灵芝多糖, 液体培养

Abstract: The effects of carbon source,nitrogen source and pH to accumulate the Ganoderma polysaccharide were studied in this paper.The results showed that the optimal carbon source,nitrogen source and pH were sucrose, peptone and pH5.0 respectively. The optimal conditions were: KH2PO4 0.1, MgSO·7H2O 0.05 and (NH4)2SO4 0.2, peptone 0.3, sucrose 5 and 4 pH5.0.The concentration of Ganoderma polysaccharide in mycelia was 11.77%, 2.5 fold higher than that of the fruiting body and 2.2 fold higher than that of the spores.

Key words: Ganoderma lucidum, polysaccharide, liquid medium