关键词: 动物试验, 抗疲劳, 影响因素, 分析

Abstract: Basing on the basic project defined in Functional Assessment Procedure and Test Methods of Health Food, which was issued by Chinese Sanitary Department, some factors such as the feeding temperature, water temperature of swimming experiment and experiment time would impact the time of loading swimming of mice. The method combined orthogonal test L 8 (2 7 ) has been applied to research the effecting factors of experiment time, water temperature of swimming experiment, feeding temperature and their mutual action in loaded swimming of china medicine function liquidⅠ, the orders of effecting factors of loading swimming applied by extreme difference (R low) was: Water temperature of swimming experiment > experiment time > (water temperature of swimming experiment × experiment time)> feeding temperature > (experiment time × feeding temperature). The best combination in the experiment of loaded swimming of china medicine function liquidⅠwas: the test lasted for 30d, the water temperature of swimming experiment was 25℃ and the feeding temperature was 25℃. Therefore, the accuracy of loaded swimming experiment should be increased, or it would result in contingency of anti-fatigue appraisal results and unavoidably cause the phenomenon of bad reappearance.

Key words: animal experiment, anti-fatigue, effecting factors, analysis