食品科学 ›› 2005, Vol. 26 ›› Issue (9): 134-137.

• 基础研究 • 上一篇    下一篇

枯草杆菌纤溶酶高产菌株的物理化学诱变

 吕凤霞, 陆兆新, 别小妹, 梁思宇   

  1. 南京农业大学食品科技学院
  • 出版日期:2005-09-15 发布日期:2011-09-20

Breeding of Bacillis Subtilis Strain Produced High Fibrinolysin Activity by Induced-mutation with γ-ray and Diethyl Sulfate

 LU Feng-Xia , LU  Zhao-Xin, BIE  Xiao-Mei, LIANG  Si-Yu   

  1. College of Food Science and Technology, Nanjing Agricultural University
  • Online:2005-09-15 Published:2011-09-20

摘要: 以枯草杆菌FM-S2作为出发菌株,经γ-射线和2.5%硫酸二乙酯复合诱变,结合牛奶蛋白平板从2341株存活菌中初筛得到200株诱变菌株,经摇瓶测定纤溶酶活性进行复筛得到7株生产性能较出发菌株显著提高的突变株,它们的纤溶酶活性超过2000UK.U/ml。其中突变株γ-DES36纤溶酶活性最高达到2719.89±242.51UK.U/ml,同时该菌株遗传性能稳定。

关键词: 枯草杆菌, 纤溶酶, 辐射, 硫酸二乙酯, 突变株

Abstract: Bacillus Subtilis FM-S2, as parental strain, was mutagenized withγ-ray and DES. 200 mutants were selected by the isolation on the milk protein plates from 2341 survival.mutants. Seven of mutants over 2000UK.U/ml were obtained by measuring the fibrinolysin activity. It was found that γ-DES36 strain had the highest fibrinolysin activity, which reached 2719.89±242.51UK.U/ml, and that it was stable for 10 generations.

Key words: Bacillus Subtilis, fibrinolysin, radiation, DES, mutants