食品科学 ›› 2005, Vol. 26 ›› Issue (9): 418-419.

• 分析检测 • 上一篇    下一篇

紫外分光光度法测定剑麻花中总黄酮的含量

 刘玉芬, 夏海涛, 杨树平   

  1. 淮海工学院化学工程系
  • 出版日期:2005-09-15 发布日期:2011-09-20

Quantitative Determination of Total Flavonoids in Sisal Flower by UV Spectrophotometry

 LIU  Yu-Fen, XIA  Hai-Tao, YANG  Shu-Ping   

  1. Department of Chemical Engineering,Huaihai Institute of Technology
  • Online:2005-09-15 Published:2011-09-20

摘要: 以芦丁为标样,对剑麻花中黄酮类化合物采用甲醇超声提取,用紫外分光光度法测定,结果表明,在选定的实验条件下,芦丁的浓度和吸光度呈良好的线性关系,标准曲线的相关系数为0.9997,测定的平均回收率为98.9%,RSD=0.07%。该方法简便、快速、准确。样品提取后不经分离可直接稀释测定。

关键词: 剑麻花, 总黄酮, 紫外分光光度法

Abstract: Using rutin as the standard sample, the flavonoids substances in sisal flower were determined through methyl alcohol ultrasonic extraction and ultraviolet spectrophotometer. The determination result shows : under the chosen experiment conditions, there was a linear relationship between the concentration of the rutin solution and absorption spectrum in the range of 1.00~30μg/ ml with correlation coefficient of 0.9980. The average recovery of this method was 98. 9%, and the relative standard deviation was0. 07%. The sample dilutes directly for determination.This analysis method develop was simple, fast and accurate.

Key words: sisal flower, total flavonoids, UV spectrophotometry