食品科学 ›› 2006, Vol. 27 ›› Issue (1): 190-192.

• 分析检测 • 上一篇    下一篇

美味牛肝菌多糖中β-葡聚糖含量的酶法测定

 李志洲, 邓百万, 杨海涛, 陈文强   

  1. 陕西理工学院化学系陕西汉中; 陕西理工学院生物系
  • 出版日期:2006-01-15 发布日期:2011-09-05

Enzymatic Assay of β-Glucans in Polysaccharides of Boletus edulis

 LI  Zhi-Zhou, DENG  Bai-Wan, YANG  Hai-Tao, CHEN  Wen-Qiang   

  1. 1.Department of Chemistry, Shaanxi University of Technology; 2.Department of Biology, Shaanxi University of Technology.
  • Online:2006-01-15 Published:2011-09-05

摘要: 本文利用全酶法对美味牛肝菌多糖中β-葡聚糖的含量进行了测定。结果表明,三种酶的最佳用量为α-淀粉酶为2000U/g、胰蛋白酶为1500U/g、糖化酶为4000U/g,测得它的β-葡聚糖的含量是5.1%。

关键词: 牛肝菌, 多糖, &beta, -葡聚糖,

Abstract:  An enzymatic method for analysis of technology in polysaccharides of Boletus edulis Bull has been studied in this paper .The experimental results showed that the optimal amount of three enzymes used were: α-amylase 2000U/g,trypsin 1500U/ g and saccharogenic enzyme 4000U/g. The yield of the sample obtained was 5.1% content of β-glucans.

Key words: Boletus edulis; paysaccharides; &beta, - glucan; enzyme;